MBE Advance Access published online on April 6, 2009
Molecular Biology and Evolution, doi:10.1093/molbev/msp065
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Research Article |
Recent strong positive selection on Drosophila melanogaster HDAC6, a gene encoding a stress surveillance factor, as revealed by population genomic analysis
Co-authors affiliations: Pavlos Pavlidis: pavlidis{at}bio.lmu.de, Section of Evolutionary Biology, LMU BioCenter, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany, Tel: +49 89 2180 74 101, Fax: +49 89 2180 74 104
Prof. Dr. Wolfgang Stephan: stephan{at}bio.lmu.de, Section of Evolutionary Biology, LMU BioCenter, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany, Tel: +49 89 2180 74 101, Fax: +49 89 2180 74 104
Corresponding author: Nicolas Svetec: svetec{at}bio.lmu.de, Section of Evolutionary Biology, LMU BioCenter, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany, Tel: +49 89 2180 74 101, Fax: +49 89 2180 74 104
Received for publication December 20, 2008. Revision received February 19, 2009. Accepted for publication March 26, 2009.
Based on nearly complete genome sequences from a variety of organisms, data on naturally occurring genetic variation on the scale of hundreds of loci to entire genomes have been collected in recent years. In parallel, new statistical approaches (such as the CLR and SweepFinder tests) have been developed to infer evidence of recent positive selection from these data and to localize the target of selection. Here we apply these methods to the X chromosome of Drosophila melanogaster in an effort to map genes involved in ecological adaptation. Using slight modifications of these tests that increase their robustness against past demographic changes, we detected evidence that recent strongly positive selection has been acting on a 2.7-kb region in an ancestral African population. This region overlaps with the 3’ end of HDAC6, a gene that encodes a newly characterized stress surveillance factor. HDAC6 is an unusual histone deacetylase being localized in the cytoplasm. Its ubiquitin-binding and tubulin-deacetylase activities suggest that HDAC6 is very different from other histone deacetylases. Indeed, recent discoveries have shown that HDAC6 is a key regulator of cytotoxic stress resistance.
Key Words: Drosophila melanogaster • selective sweep • selection mapping • HDAC6