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MBE Advance Access published online on January 10, 2008
Molecular Biology and Evolution, doi:10.1093/molbev/msm276
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© The Author 2008. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Research Article

Hydrogen Peroxide-Induced Gene Expression across Kingdoms: A Comparative Analysis

Korneel Vandenbroucke*,{dagger}, Steven Robbens*,{dagger}, Klaas Vandepoele*,{dagger}, Dirk Inzé*,{dagger}, Yves Van de peer*,{dagger} and Frank Van Breusegem*,{dagger}

* Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium
{dagger} Department of Molecular Genetics, Ghent University, B-9052 Ghent, Belgium

Mailing address: Frank Van Breusegem, Department Plant Systems Biology, VIB, Universiteit Gent, Technologiepark 927, B-9052 Gent (Belgium) tel. 32-9-3313800; fax 32-9-3313809; e-mail frank.vanbreusegem{at}psb.ugent.be

Received for publication October 29, 2007. Revision received November 30, 2007. Accepted for publication December 7, 2007.

Cells react to oxidative stress conditions by launching a defense response through the induction of nuclear gene expression. The advent of microarray technologies allowed monitoring of oxidative stress-dependent changes of transcript levels at a comprehensive and genome-wide scale, resulting in a series of inventories of differentially expressed genes in different organisms. We performed a meta-analysis on hydrogen peroxide (H2O2)-induced gene expression in the cyanobacterium Synechocystis PCCG6803, the yeast Saccharomyces cerevisiae and Schizosaccharomyces pombe, the land plant Arabidopsis thaliana, and the human HeLa cell line. The H2O2-induced gene expression in both yeast species was highly conserved and more similar to the A. thaliana response than that of the human cell line. Based on the expression characteristics of genuine antioxidant genes, we show that the antioxidant capacity of microorganisms and higher eukaryotes is differentially regulated. Four families of evolutionarily conserved eukaryotic proteins could be identified that were H2O2-responsive across kingdoms: DnaJ domain-containing heat shock proteins, small GTP-binding proteins, Ca2+-dependent protein kinases, and ubiquitin-conjugating enzymes.

Key Words: oxidative stress • hydrogen peroxide • microarray • comparative transcriptomics • Synechocystis • yeast • Arabidopsis • H. sapiens


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