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MBE Advance Access published online on July 13, 2007
Molecular Biology and Evolution, doi:10.1093/molbev/msm137
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© The Author 2007. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Research Article

Duplication and Selection on Abalone Sperm Lysin in An Allopatric Population

Nathaniel L. Clark*, Geoffrey D. Findlay*, Xianhua Yi, Michael J. MacCoss and Willie J. Swanson

Department of Genome Sciences, University of Washington, Seattle, WA, USA

Corresponding Authors:Nathaniel Clark and Geoffrey Findlay, Box 355065, 1705 NE Pacific St., Seattle, WA 98195-5065, phone: (206) 221-5124, email: clarknl{at}u.washington.edu, gfindlay{at}u.washington.edu, fax: (206) 685-7301

Received for publication March 27, 2007. Revision received June 18, 2007. Accepted for publication July 3, 2007.

Gene duplication is a major source of evolutionary novelty, yet the importance of this process in reproductive protein evolution has not been widely investigated. Here, we report the first known case of gene duplication of abalone sperm lysin in an allopatric subspecies found in the Eastern Atlantic, Haliotis tuberculata coccinea. Mass spectrometry identified both copies of the lysin protein in testis tissue, and three-dimensional structural modeling suggests that both proteins remain functional. We also detected positive selection acting on both paralogs after duplication and found evidence of a recent selective sweep. Because H. t. coccinea occurs in geographic isolation from other abalone species, these findings suggest that the evolution of lysin is not driven to create reproductive barriers to unfit hybrid formation with an overlapping species. Instead, sexual selection or sexual conflict acting during abalone fertilization could be responsible for the recent positive selection on this protein. The presence of multiple, rapidly evolving lysin genes in H. tuberculata presents an opportunity to study the early stages of diversification of a protein whose function is well understood.

Key Words: lysin • gene duplication • positive selection • Haliotis tuberculata • reinforcement • sexual conflict

* these authors contributed equally to this work


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