MBE Advance Access published online on June 1, 2007
Molecular Biology and Evolution, doi:10.1093/molbev/msm108
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2007 The Authors.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research Article |
Context Dependence, Ancestral Misidentification, and Spurious Signatures of Natural Selection
* Biological Statistics and Computational Biology, Cornell University, Ithaca, New York 14850 USA
Corresponding Author: Carlos D. Bustamante, Department of Biological Statistics and Computational Biology, 101 Biotechnology Building, Cornell University, Ithaca, NY 14850, phone (607)255-1640, fax (607)255-4698, cdb28{at}cornell.edu
Received for publication August 16, 2006. Revision received March 15, 2007. Revision received May 7, 2007. Accepted for publication May 11, 2007.
Population genetic analyses often use polymorphism data from one species, and orthologous genomic sequences from closely related outgroup species. These outgroup sequences are frequently used to identify ancestral alleles at segregating sites and to compare the patterns of polymorphism and divergence. Inherent in such studies is the assumption of parsimony, which posits that the ancestral state of each single nucleotide polymorphism (SNP) is the allele that matches the orthologous site in the outgroup sequence, and that all nucleotide substitutions between species have been observed. This study tests the effect of violating the parsimony assumption when mutation rates vary across sites and over time. Using a context-dependent mutation model that accounts for elevated mutation rates at CpG dinucleotides, increased propensity for transitional versus transversional mutations, as well as other directional and contextual mutation biases estimated along the human lineage, we show (using both simulations and a theoretical model) that enough unobserved substitutions could have occurred since the divergence of human and chimpanzee to cause many statistical tests to spuriously reject neutrality. Moreover, using both the chimpanzee and rhesus macaque genomes to parsimoniously identify ancestral states causes a large fraction of the data to be removed while not completely alleviating problem. By constructing a novel model of the context-dependent mutation process, we can correct polymorphism data for the effect of ancestral misidentification using a single outgroup.
Key Words: context-dependence • single nucleotide polymorphism • site-frequency spectrum • ancestral misidentification
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. E. Lohmueller, C. D. Bustamante, and A. G. Clark Methods for Human Demographic Inference Using Haplotype Patterns From Genomewide Single-Nucleotide Polymorphism Data Genetics, May 1, 2009; 182(1): 217 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Hernandez A flexible forward simulator for populations subject to selection and demography Bioinformatics, December 1, 2008; 24(23): 2786 - 2787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Desai and J. B. Plotkin The Polymorphism Frequency Spectrum of Finitely Many Sites Under Selection Genetics, December 1, 2008; 180(4): 2175 - 2191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Foxe, V.-u.-N. Dar, H. Zheng, M. Nordborg, B. S. Gaut, and S. I. Wright Selection on Amino Acid Substitutions in Arabidopsis Mol. Biol. Evol., July 1, 2008; 25(7): 1375 - 1383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Casillas, A. Barbadilla, and C. M. Bergman Purifying Selection Maintains Highly Conserved Noncoding Sequences in Drosophila Mol. Biol. Evol., October 1, 2007; 24(10): 2222 - 2234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Hernandez, S. H. Williamson, L. Zhu, and C. D. Bustamante Context-Dependent Mutation Rates May Cause Spurious Signatures of a Fixation Bias Favoring Higher GC-Content in Humans Mol. Biol. Evol., October 1, 2007; 24(10): 2196 - 2202. [Abstract] [Full Text] [PDF]
|
|