MBE Advance Access published online on February 13, 2007
Molecular Biology and Evolution, doi:10.1093/molbev/msm030
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Research Article |
Comparative Analysis of Sequences Preceding Protein-Coding Mitochondrial Genes in Flowering Plants
Biology Department, University of Ottawa, Ottawa, Canada K1N 6N5
* Corresponding author: Dr. Linda Bonen, Biology Department, University of Ottawa, 30 Marie Curie, Ottawa, Canada K1N 6N5 lbonen{at}science.uottawa.ca, FAX: (613) 562-5486, Tel: (613) 562-5800 ext. 6356
Accepted for publication February 8, 2007.
We examined the nucleotide sequences preceding 23 mitochondrial protein-coding genes held in common by maize, rice, wheat, sugar beet, tobacco, Arabidopsis and Brassica to look for features related to translation initiation and to assess the degree of conservation in mitochondrial mRNA leaders among these plants. We observed broad variation in sequence similarity as illustrated by dot plot analysis, ranging from a level rivalling that of coding sequences to complete absence of homology due to lineage-specific DNA rearrangements. Genes encoding ATP synthase subunits predominated in the latter category whereas ones encoding cytochrome c biogenesis proteins and NADH dehydrogenase subunits were primarily of the highly-conserved type. Within the region immediately preceding initiation codons, in most cases we did not observe motifs consistent with a bacterial-type Shine-Dalgarno interaction to assist in ribosome binding, nor was any other consensus sequence evident. In fact, indels in the form of tandem repeats were seen among homologues from different plants. We did however observe a bias for high adenosine and low cytosine in the proximal 
30 nt compared to further upstream. Duplicates of some sequences in our data set were found to be associated with more than one gene within a genome. Indeed, three such families of upstream cassettes were identified and they exhibit a lineage-specific distribution among plants. Moreover, the presence of related sequences at genomic sites distant from known genes raises the possibility of future recruitment as regulatory elements. Our observations point to a dynamic nature in the make-up of the 5' leaders of plant mitochondrial mRNAs and an apparent plasticity in translational control elements.
Key Words: plant mitochondria, 5' • UTR, translation initiation • DNA rearrangement
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