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MBE Advance Access published online on October 10, 2006
Molecular Biology and Evolution, doi:10.1093/molbev/msl142
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© The Author 2006. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Accepted September 29, 2006

Research Article

Genome-Wide Patterns of Expression in Drosophila Pure Species and Hybrid Males. II. Examination of Multiple Species Hybridizations, Platforms, and Life-Cycle Stages

Amanda J. Moehring 1, Katherine C. Teeter 1, and Mohamed A. F. Noor 1 *

1 DCMB Group/ Biology, Duke University, Box 91000, Durham, NC 27708 USA

* To whom correspondence should be addressed.
Mohamed A. F. Noor, E-mail: NOOR{at}DUKE.EDU


  Abstract

Species often produce sterile hybrids early in their evolutionary divergence, and some evidence suggests that hybrid sterility may be associated with deviations or disruptions in gene expression. In support of this idea, many studies have shown that a high proportion of male-biased genes are underexpressed compared to non-sex-biased genes in sterile F1 male hybrids of Drosophila species. In this study, we examined and compared patterns of misexpression in sterile F1 male hybrids of Drosophila simulans and two of its sibling species, D. mauritiana and D. sechellia at both the larval and adult life stages. We analyzed hybrids using both commercial D. melanogaster microarrays and arrays we developed from RT-PCRs of spermatogenesis and reproduction-related transcripts from these species ("sperm array"). While the majority of misexpressed transcripts were underexpressed, a disproportionate number of the overexpressed transcripts were located on the X chromosome. We detected a high overlap in the genes misexpressed between the two species pairs, and our sperm array was better at detecting such misexpression than the D. melanogaster array, suggesting possible weaknesses in the use of an array designed from another species. We found only minimal misexpression in the larval samples with the sperm array, suggesting that disruptions in spermatogenesis occur after this life stage. Further study of these misexpressed loci may allow us to identify precisely where disruptions in the spermatogenesis pathway occur.

Keywords: gene regulation; Drosophila simulans; Drosophila mauritiana; Drosophila sechellia; hybrid male sterility; speciation.
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