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MBE Advance Access published online on September 20, 2006
Molecular Biology and Evolution, doi:10.1093/molbev/msl127
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© The Author 2006. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org
Accepted September 18, 2006

Research Article

Transcription and Evolutionary Dynamics of the Centromeric Satellite Repeat CentO in Rice

Hye-Ran Lee 1 *, Pavel Neumann 1 *, Jiri Macas 2, and Jiming Jiang 1 *

1 Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA
2 Institute of Plant Molecular Biology, Branisovska 31, Ceske Budejovice CZ-37005, Czech Republic

* To whom correspondence should be addressed.
Jiming Jiang, E-mail: jjiang1{at}wisc.edu


  Abstract

Satellite DNA is a major component of centromeric heterochromatin in most multicellular eukaryotes, where it is typically organized into megabase-sized tandem arrays. It has recently been demonstrated that small interfering RNAs (siRNAs) processed from centromeric satellite repeats can be involved in epigenetic chromatin modifications which appear to underpin centromere function. However, the structural organization and evolution of the centromeric satellite DNA is still poorly understood. We analyzed the centromeric satellite repeat arrays from rice chromosomes 1 and 8 and identified higher-order structures and local homogenization of the CentO repeats in these two centromeres. We also cloned the CentO repeats from the CENH3-associated nucleosomes by a chromatin immunoprecipitation (ChIP)-based method. Sequence variability analysis of the ChIPed CentO repeats revealed a single variable domain within the repeat. We detected transcripts derived from both strands of the CentO repeats. The CentO transcripts are processed into siRNA, suggesting a potential role of this satellite repeat family in epigenetic chromatin modification.

*These two authors contributed equally to this work.


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