Skip Navigation



MBE Advance Access published online on August 31, 2005
Molecular Biology and Evolution, doi:10.1093/molbev/msj002
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
23/1/10    most recent
msj002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Olinski, R. P.
Right arrow Articles by Hallböök, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Olinski, R. P.
Right arrow Articles by Hallböök, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Accepted July 18, 2005

Research Article

Conserved Synteny Between the Ciona Genome and Human Paralogons Identifies Large Duplication Events in the Molecular Evolution of the Insulin-Relaxin Gene Family

Robert Piotr Olinski 1, Lars-Gustav Lundin 2, and Finn Hallböök 1*

1 Department of Neuroscience, Unit of Developmental Neuroscience, Uppsala University, Uppsala, Sweden
2 Department of Neuroscience, Unit of Pharmacology, Uppsala University, Uppsala, Sweden

* To whom correspondence should be addressed.
Finn Hallböök, E-mail: Finn.Hallbook{at}neuro.uu.se


  Abstract

The aims of the study were to outline the sequence of events that gave rise to the vertebrate insulin-relaxin gene family and the chromosomal regions in which they reside. We analyzed the gene content surrounding the human insulin/relaxin genes with respect to what family they belonged to and if the duplication history of investigated families parallels the evolution of the insulin-relaxin family members. Markov Clustering and phylogenetic analysis were used to determine family identity. More than 15% of the genes belonged to families that have paralogs in the regions, defining two sets of quadruplicate paralogy-regions. Thereby, the localization of insulin/relaxin genes in human is in accordance with that regions on human chromosomes 1, 11, 12, 19q (insulin/IGFs) and 1, 6p/15q, 9/5, 19p (INSLs/relaxins) were formed during two genome duplications. We compared the human genome with that of Ciona intestinalis, a species that split from the vertebrate lineage before the two suggested genome duplications. Two insulin-like orthologs were discovered in addition to the already described Ci-insulin gene. Conserved synteny between the Ciona regions hosting the insulin-like genes and the two sets of human paralogons implies their common origin. Linkage of the two human paralogons, as seen in human chromosome 1, as well as the two regions hosting the Ciona insulin-like genes, suggests that a segmental duplication gave rise to the entire region prior to the genome doublings. Thus, preserved gene content provides support that genome duplication(s) in addition to segmental and single gene duplications shaped the genomes of extant vertebrates.

Keywords: insulin; relaxin; gene duplication; paralogous region; Ciona intestinalis; conserved synteny.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome ResHome page
L. Z. Holland, R. Albalat, K. Azumi, E. Benito-Gutierrez, M. J. Blow, M. Bronner-Fraser, F. Brunet, T. Butts, S. Candiani, L. J. Dishaw, et al.
The amphioxus genome illuminates vertebrate origins and cephalochordate biology
Genome Res., July 1, 2008; 18(7): 1100 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
W. Wang, J. Zhong, B. Su, Y. Zhou, and Y.-Q. Wang
Comparison of Pax1/9 Locus Reveals 500-Myr-Old Syntenic Block and Evolutionary Conserved Noncoding Regions
Mol. Biol. Evol., March 1, 2007; 24(3): 784 - 791.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.