MBE Advance Access published online on August 29, 2003
Molecular Biology and Evolution, doi:10.1093/molbev/msg221
Molecular Biology and Evolution © Society for Molecular Biology and Evolution 2003; all rights reserved
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Institut Jacques Monod, Universités Paris 6 et 7, CNRS, Laboratoire Dynamique du Génome et Evolution, 4, place Jussieu, 75252, Paris Cedex 05, France
* To whom correspondence should be addressed. E-mail: higuet{at}ccr.jussieu.fr.
The hobo transposable element of Drosophila melanogaster is known to induce a hybrid dysgenesis syndrome, moreover it displays a polymorphism of a microsatellite in its coding region: TPE repeats. In European populations, surveys of the distribution of hobo elements with regard to TPE repeats revealed that the 5TPE element is distributed along a frequency gradient, and it is even more frequent than the 3TPE element in Western populations. This suggests that the invasive ability of the hobo elements could be related to the number of TPE repeats they contain. To test this hypothesis we monitored the evolution of 16 lines derived from 5 initial independent transgenic lines bearing the 3TPE element and/or the 5TPE element. Four lines bearing 5TPE elements and four bearing 3TPE elements were used as a non-competitive genetic background to compare the evolution of the 5TPE element to that of the 3TPE element. Eight lines bearing both elements provided a competitive genetic context to study potential interactions between these two elements. We studied genetic and molecular aspects of the first twenty generations. At the molecular level, we showed that the 5TPE element is able to spread within the genome at least as efficiently as the 3TPE element. Surprisingly, at the genetic level we found that the 5TPE element is less active than the 3TPE element, and moreover may be able to regulate the activity of the 3TPE element. Our findings suggest that the invasive potential of the 5TPE element could be due not only to its intrinsic transposition capacity, but also to a regulatory potential. Key Words:
Hybrid dysgenesis, hobo element, permissivity, microsatellite
© 2003 Society for Molecular Biology and Evolution
Original Articles
Behavior of the Hobo Transposable Element with Regard to TPE Repeats in Transgenic Lines of Drosophila melanogaster
2 CNRS, Laboratoire Population, Génétique et Evolution, Avenue de la Terrasse, Bâtiment 13, 91198, Gif sur Yvette, France
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. A. Subramanian, L. A. Cathcart, E. S. Krafsur, P. W. Atkinson, and D. A. O'Brochta Hermes Transposon Distribution and Structure in Musca domestica J. Hered., July 1, 2009; 100(4): 473 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Rouzic and P. Capy Population Genetics Models of Competition Between Transposable Element Subfamilies Genetics, October 1, 2006; 174(2): 785 - 793. [Abstract] [Full Text] [PDF]
|
|