MBE Advance Access published online on June 27, 2003
Molecular Biology and Evolution, doi:10.1093/molbev/msg173
Molecular Biology and Evolution © Society for Molecular Biology and Evolution 2003; all rights reserved
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1 Department of Biological Sciences, Stanford University, Stanford, CA 94305; Department of Field Crops and Genetics, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
* To whom correspondence should be addressed. E-mail: liord{at}stanford.edu.
Genome duplications may have played a role in the early stages of vertebrate evolution, near the time of divergence of the lamprey lineage. Additional genome duplication, specifically in ray-finned fish, may have occurred before the divergence of the teleosts. The common carp (Cyprinus carpio) has been considered tetraploid because of its chromosome number (2n = 100) and its high DNA content. We studied variation using 59 microsatellite primer pairs to better understand the ploidy level of the common carp. Based on the number of PCR amplicons per individual, about 60% of these primer pairs are estimated to amplify duplicates. Segregation patterns in families suggested a partially duplicated genome structure and disomic inheritance. This could suggest that the common carp is tetraploid and that polyploidy occurred by hybridization (allotetraploidy). From sequences of microsatellite flanking regions, we estimated the difference per base between pairs of alleles and between pairs of paralogues. The distribution of differences between paralogues had two distinct modes suggesting one whole genome duplication and a more recent wave of segmental duplications. The genome duplication was estimated to have occurred about 12 mya, and the segmental duplications occurring between 2.3 and 6.8 mya. At twelve mya, this would be one of the most recent genome duplications among vertebrates. Phylogenetic analysis of several cyprinid species suggests an evolutionary model for this tetraploidization with a role for polyploidization in speciation and diversification. Key Words:
Duplication, Polyploidy, Segregation, Genome evolution, Diversification, Carp
© 2003 Society for Molecular Biology and Evolution
Original Articles
Recent Duplication of the Common Carp (Cyprinus carpio L.) Genome as Revealed by Analyses of Microsatellite Loci
2 Department of Field Crops and Genetics, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
3 Department of Biological Sciences, Stanford University, Stanford, CA 94305
4 Institute of Horticulture, ARO-Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
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