MBE Advance Access published online on April 2, 2003
Molecular Biology and Evolution, doi:10.1093/molbev/msg080
Molecular Biology and Evolution © Society for Molecular Biology and Evolution 2003; all rights reserved
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1 Department of Anatomy and Cell Biology, Wayne State University
* To whom correspondence should be addressed. E-mail: wmoore{at}biology.biosci.wayne.edu.
We sequenced Key Words:
Fibrinogen intron, intron alignment, bird, Aves, conserved intron sequence, avian phylogeny
© 2003 Society for Molecular Biology and Evolution
Original Articles
Alignment and Phylogenetic Analysis of
-Fibrinogen Intron 7 Sequences among Avian Orders Reveal Conserved Regions within the Intron
2 Department of Biological Sciences, Wayne State University
Abstract 
-fibrinogen intron 7 (-fibint7) from 28 species of birds, representing 18 families in nine orders. Although the antiquity of the avian orders is estimated to be 55-90 MY, and numerous indels have accrued among diverging lineages, the intron sequences were not difficult to align. However, alignment of avian sequences with mammal or snake sequences was difficult and the residual phylogenetic signal weak. -fibint 7 is an AT-rich intron, and its base composition varies little over the diversity of birds represented by our sample. Alignment of these anciently diverged sequences reveals at least five clusters of conserved nucleotides; at least two clusters appear to be in excess of the minimal set usually associated with intron excision, but their functions are unknown. Two equally most-parsimonious (MP) trees were found when indels were not included in the phylogenetic analysis and six when they were included. The neighbor-joining and maximum likelihood trees were identical to each other and to one of the MP trees in each MP analysis. Indels, as well as nucleotide substitutions, are phylogenetically informative and bootstrap support exceeded 90% for 21 of 24 inferred nodes when indels were included in the MP analysis. All traditional orders represented by two or more species appear monophyletic. Relationships among avian orders are strongly supported with the exception of an inferred sister-group relationship between Caprimulgiformes and Columbiformes. A relatively close relationship between Piciformes and Passeriformes is inferred, at odds with earlier DNA-DNA hybridization studies but consistent with traditional classifications. Among Passeriformes, the traditional perspective of a sister-group relationship of suboscines and oscines is supported as is the subsequent split of the oscines into a lineage representative of the Corvida before the diversification of the Passerida. The four species of owls divide into two strongly supported clades, corresponding to the widely accepted bifurcation of owls into two families, Tytonidae and Strigidae. A sister-group relationship between gallinaceous birds and waterfowl, the Galloanserae, is also strongly supported.
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