MBE Advance Access published online on February 4, 2003
Molecular Biology and Evolution, doi:10.1093/molbev/msg035
Molecular Biology and Evolution © Society for Molecular Biology and Evolution 2003; all rights reserved
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1 Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
* To whom correspondence should be addressed. E-mail: Doris.Bachtrog{at}ed.ac.uk.
Transposable elements constitute a major fraction of eukaryotic genomes. Here, I characterize two novel non-LTR retrotransposons, cloned from the neo-Y chromosome of Drosophila miranda. worf is 4,1 kb in size and shows homology to the T1-2 non-LTR transposon characterized in Anopheles. spock is 4,9 kb in size and shows similarity to the Doc element of D. melanogaster. Southern blot analysis of both elements yielded stronger signals for male DNA. In situ hybridization to polytene chromosomes revealed that both elements are accumulating on the neo-Y chromosome of D. miranda. PCR analysis was conducted to investigate the frequency of spock and worf, and of the previously identified transposons TRIM and TRAM, at individual chromosomal sites among twelve strains of D. miranda. Contrary to the observation that element frequencies are usually kept low at individual sites in Drosophila, the four transposons investigated are fixed at their genomic locations on the neo-Y chromosome. These results support the hypothesis that TEs accumulate in non-recombining regions, and may be one cause of the heteromorphism of sex chromosomes. Key Words:
Keywords: Y chromosome degeneration, transposable elements, Drosophila, heterochromatin
© 2003 Society for Molecular Biology and Evolution
Original Articles
Accumulation of spock and worf, two novel non-LTR retrotransposons, on the neo-Y chromosome of Drosophila miranda
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