Skip Navigation


MBE Advance Access originally published online on July 29, 2008
Molecular Biology and Evolution 2008 25(10):2129-2139; doi:10.1093/molbev/msn164
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
25/10/2129    most recent
msn164v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Omilian, A. R.
Right arrow Articles by Lynch, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Omilian, A. R.
Right arrow Articles by Lynch, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Research Articles

Intron Presence–Absence Polymorphisms in Daphnia

Angela R. Omilian1, Douglas G. Scofield2 and Michael Lynch

Department of Biology, Indiana University

E-mail: alr2{at}buffalo.edu.

Accepted for publication July 16, 2008.

Here, we report 2 novel intron gains segregating in populations of Daphnia pulex endemic to Oregon. These novel introns do not have an obvious source and are not present in any D. pulex populations outside Oregon, other species of Daphnia that we examined, or any other organism for which sequence data are available. Furthermore, the novel introns are both found in the same gene, a Rab GTPase (rab4), and they appear to differ in their insertion site by one base pair, providing some support to the proto-splice site hypothesis. The rarity of intron-gain polymorphisms is questioned as we discovered 2 events in an initial survey of only 6 nuclear loci in 36 Daphnia individuals. Neutrality tests failed to ascertain a clear selective effect for either intron insertion, and a significant difference in recombination rate was not observed in alleles that contain the novel intron insertion versus alleles lacking it. We conclude that one novel intron insertion segregating at high frequencies in Daphnia populations in Oregon is unlikely to be adaptive and may result from the reduced efficacy of selection in isolated populations of small effective size.

Key Words: Daphnia • intron insertion • intron gain • intron polymorphism

1 Present address: Department of Biological Sciences, University at Buffalo, Buffalo, NY 14260.

2 Present address: Department of Ecology and Evolutionary Biology, University of California, Los Angeles.

Kenneth Wolfe, Associate Editor


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J HeredHome page
F. Catania, X. Gao, and D. G. Scofield
Endogenous Mechanisms for the Origins of Spliceosomal Introns
J. Hered., September 1, 2009; 100(5): 591 - 596.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. R. Omilian and M. Lynch
Patterns of Intraspecific DNA Variation in the Daphnia Nuclear Genome
Genetics, May 1, 2009; 182(1): 325 - 336.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.