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MBE Advance Access originally published online on April 13, 2005
Molecular Biology and Evolution 2005 22(7):1531-1534; doi:10.1093/molbev/msi146
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© The Author 2005. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org

Letter

Directed Mutagenesis Confirms the Functional Importance of Positively Selected Sites in Polygalacturonase Inhibitor Protein

John G. Bishop1

Max Planck Institute For Chemical Ecology, Jena, Germany; and School of Biological Sciences, Washington State University

E-mail: bishop{at}vancouver.wsu.edu.

Polygalacturonase inhibitor proteins (PGIPs) protect plants against invasion by diverse microbial and invertebrate enemies that use polygalacturonase (PG) to breach the plant cell wall. Directed mutagenesis has identified specific natural mutations conferring novel defensive capability in green bean PGIP against a specific fungal PG. These same sites are identified as positively selected by phylogenetic codon-substitution models, demonstrating the utility of such models for connecting retrospective comparative analyses with contemporary, ecologically relevant variation.

Key Words: polygalacturonase inhibitor protein • directed mutagenesis • bean • Phaseolus vulgaris • positive selection • codon-substitution models


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