Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bettencourt, B. R.
Right arrow Articles by Feder, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bettencourt, B. R.
Right arrow Articles by Feder, M. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Biology and Evolution 18:1272-1282 (2001)
© 2001 Society for Molecular Biology and Evolution

Hsp70 Duplication in the Drosophila melanogaster Species Group: How and When Did Two Become Five?

Brian R. Bettencourt and Martin E. Feder

Department of Organismal Biology and Anatomy
Committee on Evolutionary Biology, University of Chicago

To determine how the modern copy number (5) of hsp70 genes in Drosophila melanogaster evolved, we localized the duplication events that created the genes in the phylogeny of the melanogaster group, examined D. melanogaster genomic sequence to investigate the mechanisms of duplication, and analyzed the hsp70 gene sequences of Drosophila orena and Drosophila mauritiana. The initial two-to-four hsp70 duplication occurred 10–15 MYA, according to fixed in situ hybridization to polytene chromosomes, before the origin and divergence of the melanogaster and five other species subgroups of the melanogaster group. Analysis of more than 30 kb of flanking sequence surrounding the hsp70 gene clusters suggested that this duplication was likely a retrotransposition. For the melanogaster subgroup, Southern hybridization and an hsp70 restriction map confirmed the conserved number (4) and arrangement of hsp70 genes in the seven species other than D. melanogaster. Drosophila melanogaster is unique; tandem duplication and gene conversion at the derived cluster yielded a fifth hsp70 gene. The four D. orena hsp70 genes are highly similar and concertedly evolving. In contrast, the D. mauritiana hsp70 genes are divergent, and many alleles are nonfunctional. The proliferation, concerted evolution, and maintenance of functionality in the D. melanogaster hsp70 genes is consistent with the action of natural selection in this species.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
D. Schmidt and R. Durrett
Adaptive Evolution Drives the Diversification of Zinc-Finger Binding Domains
Mol. Biol. Evol., December 1, 2004; 21(12): 2326 - 2339.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
N. Nikolaidis and M. Nei
Concerted and Nonconcerted Evolution of the Hsp70 Gene Superfamily in Two Sibling Species of Nematodes
Mol. Biol. Evol., March 1, 2004; 21(3): 498 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
D. N. Lerman, P. Michalak, A. B. Helin, B. R. Bettencourt, and M. E. Feder
Modification of Heat-Shock Gene Expression in Drosophila melanogaster Populations via Transposable Elements
Mol. Biol. Evol., January 1, 2003; 20(1): 135 - 144.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.