Molecular Biology and Evolution 18:530-541 (2001)
© 2001 Society for Molecular Biology and Evolution
ARTICLE |
Giardia lamblia Expresses a Proteobacterial-like DnaK Homolog
*The Josephine Bay Paul Center of Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts;
Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada; and
Department of Pathology, Division of Infectious Diseases and Center for Molecular Genetics, University of California at San Diego School of Medicine
We identified a novel gene encoding molecular chaperone HSP70 in the amitochondriate parasite Giardia lamblia. The predicted protein is similar to bacterial DnaK and mitochondrial HSP70s. The gene is transcribed and translated at a constant level during trophozoite growth and encystation. Alignment of the sequence with a data set of cytosolic, endoplasmic reticulum (ER), mitochondrial, and DnaK HSP70 homologs indicated that the sequence was extremely divergent and contained insertions unique to giardial HSP70s. Phylogenetic analyses demonstrated that this sequence was distinct from the cytosolic and ER forms and was most similar to proteobacterial and mitochondrial DnaKs. However, a specific relationship with the alpha proteobacterial and mitochondrial sequences was not strongly supported by phylogenetic analyses of this data set, in contrast to similar analyses of cpn60. These data neither confirm nor reject the possibility that this gene is a relic of secondary mitochondrial loss; they leave open the possibility that it was acquired in a separate endosymbiotic event.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Li, J. Luo, H. Zhou, J.-Y. Liao, L.-M. Ma, Y.-Q. Chen, and L.-H. Qu Stress-induced tRNA-derived RNAs: a novel class of small RNAs in the primitive eukaryote Giardia lamblia Nucleic Acids Res., November 1, 2008; 36(19): 6048 - 6055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Martin Embley Multiple secondary origins of the anaerobic lifestyle in eukaryotes Phil Trans R Soc B, June 29, 2006; 361(1470): 1055 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. van der Giezen, G. Leon-Avila, and J. Tovar Characterization of chaperonin 10 (Cpn10) from the intestinal human pathogen Entamoeba histolytica Microbiology, September 1, 2005; 151(9): 3107 - 3115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Regoes, D. Zourmpanou, G. Leon-Avila, M. van der Giezen, J. Tovar, and A. B. Hehl Protein Import, Replication, and Inheritance of a Vestigial Mitochondrion J. Biol. Chem., August 26, 2005; 280(34): 30557 - 30563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Best, H. G. Morrison, A. G. McArthur, M. L. Sogin, and G. J. Olsen Evolution of Eukaryotic Transcription: Insights From the Genome of Giardia lamblia Genome Res., August 1, 2004; 14(8): 1537 - 1547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Leon-Avila and J. Tovar Mitosomes of Entamoeba histolytica are abundant mitochondrion-related remnant organelles that lack a detectable organellar genome Microbiology, May 1, 2004; 150(5): 1245 - 1250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Slapeta and J. S. Keithly Cryptosporidium parvum Mitochondrial-Type HSP70 Targets Homologous and Heterologous Mitochondria Eukaryot. Cell, April 1, 2004; 3(2): 483 - 494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Cavalier-Smith The excavate protozoan phyla Metamonada Grasse emend. (Anaeromonadea, Parabasalia, Carpediemonas, Eopharyngia) and Loukozoa emend. (Jakobea, Malawimonas): their evolutionary affinities and new higher taxa Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1741 - 1758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. B. Simpson Cytoskeletal organization, phylogenetic affinities and systematics in the contentious taxon Excavata (Eukaryota) Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1759 - 1777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. B. Simpson, A. J. Roger, J. D. Silberman, D. D. Leipe, V. P. Edgcomb, L. S. Jermiin, D. J. Patterson, and M. L. Sogin Evolutionary History of "Early-Diverging" Eukaryotes: The Excavate Taxon Carpediemonas is a Close Relative of Giardia Mol. Biol. Evol., October 1, 2002; 19(10): 1782 - 1791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Silberman, A. G. B. Simpson, J. Kulda, I. Cepicka, V. Hampl, P. J. Johnson, and A. J. Roger Retortamonad Flagellates are Closely Related to Diplomonads--Implications for the History of Mitochondrial Function in Eukaryote Evolution Mol. Biol. Evol., May 1, 2002; 19(5): 777 - 786. [Abstract] [Full Text] [PDF]
|
|