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Molecular Biology and Evolution 18:893-895 (2001)
© 2001 Society for Molecular Biology and Evolution

LETTER

Microuli, a Family of Miniature Subterminal Inverted-Repeat Transposable Elements (MSITEs): Transposition Without Terminal Inverted Repeats

Zhijian TuGo,* and Stephanos P. Orphanidis{dagger}

*Department of Biochemistry, Virginia Polytechnic Institute and State University; and
{dagger}Department of Biochemistry, University of Arizona

The first Microuli element, Microuli-Aa1, was discovered as a 209-bp insertion in the 5' long terminal repeat of a retrotransposon named Mosqcopia-Aa2 (unpublished data, GenBank AY009101) in the yellow fever mosquito, Aedes aegypti. As shown in figure 1A, the Microuli insertion resulted in a TTAA target duplication, indicating past mobility of the element. To further characterize this mobile element, we screened an A. aegypti genomic library, using a digoxigenin-labeled single-stranded DNA probe generated by asymmetric PCR using the entire Microuli-Aa1 as the template. The library construction, probe labeling, and library screening procedures were as previously described (Tu 2000Citation ). The final washing stringency was 0.5 x SSC with 0.1% SDS at 55°C, which allows approximately 15%–25% mismatch (Meinkoth and Wahl 1984Citation ). Because 462 positive plaques were identified among 64,000 total plaques screened, it was estimated using a previously described calculation method (Tu 2000Citation ) that there were . . . [Full Text of this Article]

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