Molecular Biology and Evolution, Vol 9, 636-653, Copyright © 1992 by Society for Molecular Biology and Evolution
SB Lee and JW Taylor
Ribosomal DNA variation was used to study evolutionary relationships among
five fungal-like protoctistan Phytophthora species. On the basis of
morphological and ecological characteristics, four of these species-- P.
palmivora, P. megakarya, P. capsici, and P. citrophthora--were once thought
to be related. Variation within a species was extensively studied in a
fifth, outgroup species--P. cinnamomi--known, on the basis of ecological,
isozyme, and mitochondrial DNA studies, to be variable. Internal
transcribed spacer regions (ITS I, between the 18S and 5.8S rDNAs; and ITS
II, between the 5.8S and 25S rDNAs) from 27 isolates of these five species
were analyzed by polymerase chain reaction amplification and direct
sequencing. Intraspecific variability was undetected or low. Interspecific
nucleotide difference was 0.3%-14.6%, and comparisons of variable regions
permitted the evaluation of phylogenetic relationships among species. Both
neighbor-joining and parsimony analysis of ITS variability support a close
relationship between cacao isolates of P. capsici and P. citrophthora and a
common lineage for P. palmivora and P. megakarya. Large distance values
were estimated between P. cinnamomi and the other species. Inferred
relationships based on ITS variability were compared with those based on
other characters. The catalog of sequences provides the information
necessary to design taxon-specific probes potentially useful in taxonomic,
ecological, and population-level studies.
ORIGINAL ARTICLE
Phylogeny of five fungus-like protoctistan Phytophthora species, inferred from the internal transcribed spacers of ribosomal DNA
Department of Plant Biology, University of California, Berkeley.
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