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Molecular Biology and Evolution, Vol 9, 526-536, Copyright © 1992 by Society for Molecular Biology and Evolution

ORIGINAL ARTICLE

An association between ADH protein levels and polymorphic nucleotide variation in the Adh gene of Drosophila melanogaster

P Matthew, A Agrotis, AC Taylor and SW McKechnie
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.

Southern analysis of the Adh region of 212 Drosophila melanogaster lines collected from the Tahbilk winery revealed linkage disequilibrium between a 37-bp insertion [designated delta 2 by Kreitman (1983)] and the fast electrophoretic variant of alcohol dehydrogenase (ADH-F). Among these lines 34% contained the insert and encoded ADH-F, 33.5% encoded ADH-F and did not have the insert, and 32.5% encoded the slow electrophoretic variant of alcohol dehydrogenase (ADH-S). Strong linkage association between this insert and ADH-F is evident worldwide. Twenty-nine of the second chromosome lines were characterized for ADH protein quantity by using radial immunodiffusion. ADH quantity was estimated in both larvae and adult males raised in the presence and absence of alcohol supplement to each of two different food media. Analyses of variance indicated higher levels of ADH protein in larvae from lines with the insert (all ADH-F), compared with those without (both ADH-F and ADH-S), independent of either dietary alcohol or media type. No such difference in ADH quantity between insert- and noninsert- containing ADH-F lines was detected in adults, although the expected higher levels occurred in ADH-F lines compared with ADH-S lines. Given the high levels of linkage disequilibrium in the Adh region, these data suggest that either polymorphic nucleotide-site variants positively associated with delta 2 on the second chromosome or delta 2 itself increases larval levels of ADH protein.
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M. Veuille, V. Benassi, S. Aulard, and F. Depaulis
Allele-Specific Population Structure of Drosophila melanogaster Alcohol Dehydrogenase at the Molecular Level
Genetics, June 1, 1998; 149(2): 971 - 981.
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