Molecular Biology and Evolution, Vol 9, 507-525, Copyright © 1992 by Society for Molecular Biology and Evolution
S Thomas and RS Singh
Four sibling species from the melanogaster subgroup (Drosophila
melanogaster, D. simulans, D. sechellia, and D. mauritiana) were studied
for genetic divergence, by high-resolution two-dimensional protein
electrophoresis (2DE) coupled with ultrasensitive silver staining. A total
of eight tissues from larval and adult developmental stages representing
both gonadal (germ-line) and nongonadal (somatic) tissues were analyzed for
protein divergence between species. Close to 400 polypeptides (protein
spots) were scored from each tissue and species, and protein divergence was
measured on the basis of qualitative differences (presence/absence) of
protein spots in pairwise species comparisons. The observed levels of genic
divergence varied among tissues and among species. When larval hemolymph
proteins (which are known to be highly polymorphic) were excluded, there
was no evidence to suggest that either the larval or adult-stage proteins,
as a whole, are more diverged than the other; variation between different
tissues rather than between developmental stages appears to be the most
significant factor affecting genetic divergence between species. The
reproductive tissue (testis and accessory gland) showed more divergence
than did the nonreproductive tissue; D. melanogaster testis (from both
larvae and adult males) showed the highest level of divergence. In view of
the previous observation that D. simulans, D. mauritiana, and D. sechellia
show similar but significantly less reproductive isolation from each other
than from D. melanogaster, the present results suggest a correlation
between the levels of reproductive-tract-protein divergence and the degree
of reproductive isolation in these species.
ORIGINAL ARTICLE
A comprehensive study of genic variation in natural populations of Drosophila melanogaster. VII. Varying rates of genic divergence as revealed by two-dimensional electrophoresis
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
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