Molecular Biology and Evolution, Vol 9, 204-215, Copyright © 1992 by Society for Molecular Biology and Evolution
MF Smith, WK Thomas and JL Patton
Initial amplification and sequencing of a 366-bp fragment of the cytochrome
b gene by a conserved primer pair (MVZ 03 and MVZ 04) revealed a
nonfunctional copy of the gene with two deletions (one of which is 17 bp in
length and the other of which is 3 bp in length) in Chroeomys jelskii, a
South American akodontine rodent. By means of an alternative primer to MVZ
03--namely, MVZ 05--from the region of the tRNA for glutamic acid, a
functional copy of cytochrome b was subsequently amplified. Both primer
pairs amplify functional sequence when applied to purified mitochondrial
DNA (mtDNA). Restriction- endonuclease digestion of purified mtDNA from C.
jelskii did not reveal any additional sets of bands that would suggest
heteroplasmy in the mitochondrial genome. When probed with both functional
and nonfunctional gene fragments, MboI restriction digests revealed the
same pattern, providing further evidence that the nonfunctional copy must
be located in the nucleus. Observed differences in the mitochondrial and
nuclear sequences from two populations are consistent with a faster rate of
change in mtDNA than in nuclear DNA.
ORIGINAL ARTICLE
Mitochondrial DNA-like sequence in the nuclear genome of an akodontine rodent
Museum of Vertebrate Zoology, University of California, Berkeley 94720.
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