Molecular Biology and Evolution, Vol 7, 9-28, Copyright © 1990 by Society for Molecular Biology and Evolution
C Collet, KM Nielsen, RJ Russell, M Karl, JG Oakeshott and RC Richmond
Genomic clones containing sequences homologous to an esterase 6 (Est-6)
cDNA clone were isolated from a library of Drosophila melanogaster DNA.
Comparison of the genomic and cDNA sequences revealed that the Est-6 gene
comprises two exons, one of 1,387 bp and one of 248 bp, separated by a
short intron of 51 bp. Further sequencing revealed the presence of a tandem
duplication of the Est-6 gene (denoted Est-P) which also has an exon of
1,387 bp and an exon of 248 bp, separated by a short intron of 56 bp. The
two genes show similarities of 64% and 60% at the DNA and protein levels,
respectively. The coding regions of the genes are 197 bases apart, and
presumptive 5' regulatory sequences of Est-P overlap at least the 3'
noncoding region of Est-6. Transcripts homologous to Est-P were detected in
late larvae and adults of each sex, whereas Est- 6 transcripts are present
in all life stages but are predominant in adult males. This suggests
different physiological functions for the products of the two genes.
Southern and Northern blot hybridization analyses of the 20-kb region
surrounding the Est-6/Est-P duplication failed to detect any other
duplicated esterase genes, although this region is actively transcribed.
ORIGINAL ARTICLE
Molecular analysis of duplicated esterase genes in Drosophila melanogaster
Department of Biology, Indiana University, Bloomington 47405.
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