Molecular Biology and Evolution, Vol 7, 45-64, Copyright © 1990 by Society for Molecular Biology and Evolution
J La Roche, M Snyder, DI Cook, K Fuller and E Zouros
The scallop Placopecten magellanicus has the largest reported animal
mitochondrial DNA (average 35 kb) and exhibits large inter- and
intraindividual length variation owing to the varying copy number of a
repeated element. We have characterized the repeat array by using
restriction mapping and sequence analysis. The repeated element consists of
1,442 bp flanked on either side by the sequence ACTTTCC in a direct
orientation. The array contains two to eight copies of the repeated element
arranged in a direct orientation and in tandem. Only complete copies of the
element are present in the array. The repeat element contains three regions
with characteristic nucleotide sequences: a 10-bp inverted repeat shown to
extrude into a cruciform in a supercoiled DNA plasmid, a 120-bp tract rich
in G/C (70%) and adjacent to the inverted repeat, and periodically
interspersed homopolymer runs of A and T occurring near the middle of the
element which induce DNA curvature in dimeric constructs of the element.
The element appears to be unique to P. magellanicus. The structural
properties of the repeat element and its organization in an array of
repeats may be important in explaining the generation and maintenance of
large-scale mitochondrial DNA size variation observed in many animal
species.
ORIGINAL ARTICLE
Molecular characterization of a repeat element causing large-scale size variation in the mitochondrial DNA of the sea scallop Placopecten magellanicus
Department of Biology, Dalhousie University, Halifax, Nova Scotia.
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