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Molecular Biology and Evolution, Vol 6, 258-269, Copyright © 1989 by Society for Molecular Biology and Evolution


ORIGINAL ARTICLE

DNA fingerprints from hypervariable mitochondrial genotypes

JC Avise, BW Bowen and T Lamb
Department of Genetics, University of Georgia, Athens 30602.

Conventional surveys of restriction-fragment polymorphisms in mitochondrial DNA of menhaden fish (Brevoortia tyrannus/patronus complex) and chuckwalla lizards (Sauromalus obesus) revealed exceptionally high levels of genetic variation, attributable to differences in mtDNA size as well as in restriction sites. The observed probabilities that any two randomly drawn individuals differed detectably in mtDNA genotype were 0.998 and 0.983 in the two species, respectively. Thus, the variable gel profiles provided unique mtDNA "fingerprints" for most conspecific animals assayed. mtDNA fingerprints differ from nuclear DNA fingerprints in several empirical respects and should find special application in the genetic assessment of maternity.
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