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Molecular Biology and Evolution, Vol 6, 227-242, Copyright © 1989 by Society for Molecular Biology and Evolution


ORIGINAL ARTICLE

Phylogeny of some Fusarium species, as determined by large-subunit rRNA sequence comparison

J Guadet, J Julien, JF Lafay and Y Brygoo
Laboratoire de Cryptogamie, Universite Paris Sud, France.

Fifty-two strains from eight species of Fusarium were analyzed by rapid rRNA sequencing. Two highly variable stretches (138 and 214 nucleotides) of the 5' end of the 28S-like rRNA molecule were sequenced. Such stretches permit evaluation of the divergence between closely related species and even between varieties within a species. The phylogenetic tree computed from the number of nucleotide differences shows seven Fusarium species to be more closely related to one another than the eighth species, F. nivale, is to them. On the basis of these data, we discuss both the phylogenetic value of taxonomical criteria and the impact of our findings on the demarcation of the genus Fusarium. We conclude that this method is suitable for establishing a precise phylogeny between closely related species within a genus.
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