Molecular Biology and Evolution, Vol 6, 213-225, Copyright © 1989 by Society for Molecular Biology and Evolution
TN Nguyen, QG Phan, LP Duong, KP Bertrand and RE Lenski
We have been examining the consequences of alternative modes of regulation
of plasmid-borne, Tn10-encoded tetracycline resistance for the fitness of
Escherichia coli. In a tetracycline-free environment, we measured the
effects on fitness that were caused by (1) maximally induced expression of
the resistance operon, (2) low-level constitutive expression of the
resistance protein, (3) residual expression of the repressed resistance
operon, (4) carriage of the resistance operon, (5) the remainder of the
plasmid genome, and (6) hyperexpression of the repressor protein. We
observed large reductions in fitness that were associated with induction
and with constitutive expression of the tetracycline-resistance protein,
but there was no discernible effect of hyperexpression of the repressor
protein. We also observed a small reduction in fitness associated with the
remainder of the plasmid genome. However, any reductions in fitness that
were caused by residual expression and by carriage of the repressed operon
were not more than 0.3%. We conclude that tight gene regulation has
eliminated antagonistic pleiotropic effects of the resistance gene on
fitness, so that possession of an inducible Tn10-encoded
tetracycline-resistance operon imposes essentially no burden in the absence
of antibiotic.
ORIGINAL ARTICLE
Effects of carriage and expression of the Tn10 tetracycline-resistance operon on the fitness of Escherichia coli K12
Department of Ecology and Evolutionary Biology, University of California, Irvine 92717.
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