Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Vincek, V.
Right arrow Articles by Klein, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vincek, V.
Right arrow Articles by Klein, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Biology and Evolution, Vol 4, 483-491, Copyright © 1987 by Society for Molecular Biology and Evolution

ORIGINAL ARTICLE

Evolutionary expansion of Mhc class I loci in the mole-rat, Spalax ehrenbergi

V Vincek, D Nizetic, M Golubic, F Figueroa, E Nevo and J Klein
Max-Planck-Institut fur Biologie, Abteilung Immungenetik, Tubingen, Federal Republic of Germany.

A cosmid genomic library was prepared from a single individual of the rodent Spalax ehrenbergi, the mole rat, captured in Israel. The library was screened with a mouse probe hybridizing with all mouse class I major-histocompatibility-complex (Mhc) genes; the cross-hybridizing clones were isolated; and their restriction maps were prepared using five enzymes. A total of 93 class I-bearing clones could be identified in the library. Forty-five of these clones showed partial overlaps and could be arranged into 14 clusters. Eleven of these clusters could be shown to contain two class I genes each; the remaining clusters, as well as most of the non-overlapping clones, each contained one class I gene. After the elimination of clones with possible cloning artifacts and of clones that may carry allelic forms of a given gene in the heterozygous animal, the total number of class I loci identified in Spalax is approximately 65. The high number of loci probably arose from the duplication of either the entire class I set or the different class I families. The high number of gene copies might represent a means of selecting different functional genes from the family in different mammalian orders. Three of the approximately 65 Spalax class I genes cross-hybridize with a probe specific for the mouse K, D, and L genes; two of these genes are in the same cluster. These three elements might possibly be the functional class I genes of the mole rat.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Hao, J. Klein, and M. Nei
Heterogeneous but conserved natural killer receptor gene complexes in four major orders of mammals
PNAS, February 28, 2006; 103(9): 3192 - 3197.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.