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Molecular Biology and Evolution, Vol 3, 449-458, Copyright © 1986 by Society for Molecular Biology and Evolution

ORIGINAL ARTICLE

Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli

IP Crawford, A Wilde, EM Yelverton, D Figurski and RW Hedges
Department of Microbiology, University of Iowa, Iowa City 52242.

The genes for the large and small subunits of anthranilate synthase (trpE and trpG, respectively) have been cloned from Pseudomonas aeruginosa PAC174 into E. coli by R-prime formation with the broad-host- range plasmid R68.44. Sequential subcloning into plasmid vectors reduced the active Pseudomonas DNA fragment to a length of 3.1 kb. We obtained evidence that this region contains the promoter for its own expression and retains a vestigial regulatory response to tryptophan scarcity or excess.
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