Molecular Biology and Evolution, Vol 3, 418-426, Copyright © 1986 by Society for Molecular Biology and Evolution
M Nei and T Gojobori
Two simple methods for estimating the numbers of synonymous and
nonsynonymous nucleotide substitutions are presented. Although they give no
weights to different types of codon substitutions, these methods give
essentially the same results as those obtained by Miyata and Yasunaga's and
by Li et al.'s methods. Computer simulation indicates that estimates of
synonymous substitutions obtained by the two methods are quite accurate
unless the number of nucleotide substitutions per site is very large. It is
shown that all available methods tend to give an underestimate of the
number of nonsynonymous substitutions when the number is large.
ORIGINAL ARTICLE
Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions
Center for Demographic and Population Genetics, University of Texas at Houston 77225.
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