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MBE Advance Access originally published online on August 26, 2008
Molecular Biology and Evolution 2008 25(11):2251-2254; doi:10.1093/molbev/msn188
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© The Author 2008. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Letters

Intragenomic Variation of Fungal Ribosomal Genes Is Higher than Previously Thought

Uwe K. Simon* and Michael Weiß{dagger}

* Lehrstuhl für Pharmazeutische Biologie, Universität Würzburg, Würzburg, Germany
{dagger} Lehrstuhl Spezielle Botanik und Mykologie, Universität Tübingen, Tübingen, Germany

E-mail: uwe.simon{at}biozentrum.uni-wuerzburg.de.

Accepted for publication August 21, 2008.

Nuclear ribosomal genes in most eukaryotes are present in multiple copies and often used for taxonomic and phylogenetic analyses. We comprehensively examined intragenomic polymorphism levels of three nuclear ribosomal loci for four important plant pathogenic fungi by polymerase chain reaction amplification and cloning. Here, we show that single nucleotide polymorphisms are present in an unexpectedly high amount. This might have implications for studies of fungal evolution, phylogenetics, and population genetics. Furthermore, our work demonstrates that the majority of all ribosomal sequences obtained from one individual and gene is identical to the majority rule consensus sequence of all detected sequence variants. Due to the large number of polymorphisms found and the fact that the polymorphism level differed markedly even between ribosomal genes of one and the same individual, we assume that nuclear ribosomal genes might not always evolve in a strictly concerted manner.

Key Words: ascomycetes • cloning • evolution • majority rule consensus sequence • intragenomic polymorphism • Taq polymerase test

Geoffery McFadden, Associate Editor


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