MBE Advance Access originally published online on March 10, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Mol. Biol. Evol. 21(6):1042-1056. 2004
DOI: 10.1093/molbev/msh103
© 2004 by the Society for Molecular Biology and Evolution. ISSN: 0737-4038
Evolution of Sarcomeric Myosin Heavy Chain Genes: Evidence from Fish
* Institute of Neurosciences
Center for Ecology and Evolutionary Biology, University of Oregon
E-mail: mcguigan{at}darkwing.uoregon.edu
Myosin heavy chain (MYH) is a major structural protein, integral to the function of sarcomeric muscles. We investigated both exon-intron organization and amino acid sequence of sarcomeric MYH genes to infer their evolutionary history in vertebrates. Our results were consistent with the hypothesis that a multigene family encoded MYH proteins in the ancestral chordate, one gene ancestral to human MYH16 and its homologues and another ancestral to all other vertebrate sarcomeric MYH genes. We identified teleost homologues of mammalian skeletal and cardiac MYH genes, indicating that the ancestors of those genes were present before the divergence of actinopterygians and sarcopterygians. Indeed, the ancestral skeletal genes probably duplicated at least once before the divergence of teleosts and tetrapods. Fish homologues of mammalian skeletal MYH are expressed in skeletal tissue and homologues of mammalian cardiac genes are expressed in the heart but, unlike mammals, there is overlap between these expression domains. Our analyses inferred two other ancestral vertebrate MYH genes, giving rise to human MYH14 and MYH15 and their homologues. Relative to the skeletal and cardiac genes, MYH14 and MYH15 homologues are characterized by evolution of intron position, differences in evolutionary rate between the functionally differentiated head and rod of the myosin protein, and possible evolution of function among vertebrate classes. Tandem duplication and gene conversion appear to have played major roles in the evolution of at least cardiac and skeletal MYH genes in fish. One outcome of this high level of concerted evolution is that different fish taxa have different suites of MYH genes, i.e., true orthologs do not exist.
Key Words: gene conversion • gene family • myosin • muscle • Teleost • Gasterosteus aculeatus
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. C. Rossi, C. Mammucari, C. Argentini, C. Reggiani, and S. Schiaffino Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles J. Physiol., January 15, 2010; 588(2): 353 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Elworthy, M. Hargrave, R. Knight, K. Mebus, and P. W. Ingham Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity Development, June 15, 2008; 135(12): 2115 - 2126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Ikeda, Y. Ono, P. Snell, Y. J. K. Edwards, G. Elgar, and S. Watabe Divergent evolution of the myosin heavy chain gene family in fish and tetrapods: evidence from comparative genomic analysis Physiol Genomics, December 19, 2007; 32(1): 1 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-S. Liang, A. Kobiyama, A. Shimizu, T. Sasaki, S. Asakawa, N. Shimizu, and S. Watabe Fast skeletal muscle myosin heavy chain gene cluster of medaka Oryzias latipes enrolled in temperature adaptation Physiol Genomics, April 24, 2007; 29(2): 201 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Di Biase and C. Franzini-Armstrong Evolution of skeletal type e-c coupling: a novel means of controlling calcium delivery J. Cell Biol., November 21, 2005; 171(4): 695 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H. Perry, B. C. Verrelli, and A. C. Stone Comparative Analyses Reveal a Complex History of Molecular Evolution for Human MYH16 Mol. Biol. Evol., March 1, 2005; 22(3): 379 - 382. [Abstract] [Full Text] [PDF]
|
|