Skip Navigation


MBE Advance Access originally published online on March 5, 2003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow All Versions of this Article:
20/3/338    most recent
msg046v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (55)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Koch, M. A.
Right arrow Articles by Mitchell-Olds, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koch, M. A.
Right arrow Articles by Mitchell-Olds, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Mol. Biol. Evol. 20(3):338-350. 2003
DOI: 10.1093/molbev/msg046
© 2003 by the Society for Molecular Biology and Evolution. ISSN: 0737-4038

Multiple Hybrid Formation in Natural Populations: Concerted Evolution of the Internal Transcribed Spacer of Nuclear Ribosomal DNA (ITS) in North American Arabis divaricarpa (Brassicaceae)

Marcus A. Koch*,, Christoph Dobes* and Thomas Mitchell-Olds{dagger}

* Institute of Botany, University of Agricultural Sciences Vienna, Vienna, Austria
{dagger} Department of Genetics and Evolution, Max-Planck-Institute for Chemical Ecology, Jena, Germany

DNA sequence variation of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA from Arabis holboellii, A. drummondii, and its putative hybrid A. divaricarpa was analyzed to study hybrid speciation in a species system geographically covering nearly the entire North American continent. Based on molecular systematics the investigated species are better combined under the genus Boechera. Multiple intraindividual ITS copies were detected in numerous accessions of A. divaricarpa, and, to a minor extent, in the parental taxa. Comparative phylogenetic analysis demonstrates that reticulate evolution is common. Consequently, concerted evolution of ITS regions resulted in different types of ITS fragments not only in hybrid populations but also in one of the parental taxa, A. holboellii. Hybrid formation often occurred independently at different sites and at different times, which is reflected by ITS copies resampling the original parental sequence variation in different ways. Some biogeographic structuring of genetic diversity is apparent and mirrors postglacial migration routes. Hybridization, reticulation, and apomixis are assumed to be the major forces driving speciation processes in this species complex. Analysis of conserved regions and secondary structures of the ITS region provided no evidence that, in this system, hybrid ITS evolution is predominantly driven in a particular direction. However, two regions in the ITS1 and ITS2, respectively, show higher mutation rates than expected from outgroup comparisons. Strong evidence for the occurrence of apomixis in A. holboellii and A. divaricarpa has come from pollen size measurements and estimations of pollen quality, which favor the hypothesis that A. drummondii served as paternal hybridization partner more frequently than A. holboellii.

Key Words: apomixis • Arabis • Boechera • Brassicaceae • phylogeography • concerted evolution • hybrid speciation • internal transcribed spacer


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Kantama, T. F. Sharbel, M. E. Schranz, T. Mitchell-Olds, S. de Vries, and H. de Jong
Diploid apomicts of the Boechera holboellii complex display large-scale chromosome substitutions and aberrant chromosomes
PNAS, August 28, 2007; 104(35): 14026 - 14031.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
Z. Kaplan and J. Fehrer
Molecular Evidence for a Natural Primary Triple Hybrid in Plants Revealed from Direct Sequencing
Ann. Bot., June 1, 2007; 99(6): 1213 - 1222.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Koch and M. Matschinger
Evolution and genetic differentiation among relatives of Arabidopsis thaliana
PNAS, April 10, 2007; 104(15): 6272 - 6277.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
M. A. Koch, C. Dobes, C. Kiefer, R. Schmickl, L. Klimes, and M. A. Lysak
Supernetwork Identifies Multiple Events of Plastid trnF(GAA) Pseudogene Evolution in the Brassicaceae
Mol. Biol. Evol., January 1, 2007; 24(1): 63 - 73.
[Abstract] [Full Text] [PDF]

Home page
 MycologiaHome page
Q.-M. Zhou, S.-Y. Guo, M.-R. Huang, and J.-C. Wei
A study of the genetic variability of Rhizoplaca chrysoleuca using DNA sequences and secondary metabolic substances.
Mycologia, January 1, 2006; 98(1): 57 - 67.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
M. E. Schranz, C. Dobes, M. A. Koch, and T. Mitchell-Olds
Sexual reproduction, hybridization, apomixis, and polyploidization in the genus Boechera (Brassicaceae)
Am. J. Botany, November 1, 2005; 92(11): 1797 - 1810.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. P. LORENZ-LEMKE, V. C. MUSCHNER, S. L. BONATTO, A. C. CERVI, F. M. SALZANO, and L. B. FREITAS
Phylogeographic Inferences Concerning Evolution of Brazilian Passiflora actinia and P. elegans (Passifloraceae) Based on ITS (nrDNA) Variation
Ann. Bot., April 1, 2005; 95(5): 799 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
M. A. Lysak, M. A. Koch, A. Pecinka, and I. Schubert
Chromosome triplication found across the tribe Brassiceae
Genome Res., April 1, 2005; 15(4): 516 - 525.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Kovarik, J. C. Pires, A. R. Leitch, K. Y. Lim, A. M. Sherwood, R. Matyasek, J. Rocca, D. E. Soltis, and P. S. Soltis
Rapid Concerted Evolution of Nuclear Ribosomal DNA in Two Tragopogon Allopolyploids of Recent and Recurrent Origin
Genetics, February 1, 2005; 169(2): 931 - 944.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
C. Dobes, T. Mitchell-Olds, and M. A. Koch
Intraspecific diversification in North American Boechera stricta (= Arabis drummondii), Boechera xdivaricarpa, and Boechera holboellii (Brassicaceae) inferred from nuclear and chloroplast molecular markers--an integrative approach
Am. J. Botany, December 1, 2004; 91(12): 2087 - 2101.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. Lihova, J. Fuertes Aguilar, K. Marhold, and G. Nieto Feliner
Origin of the disjunct tetraploid Cardamine amporitana (Brassicaceae) assessed with nuclear and chloroplast DNA sequence data
Am. J. Botany, August 1, 2004; 91(8): 1231 - 1242.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
K. MARHOLD, J. LIHOVA, M. PERNY, and W. BLEEKER
Comparative ITS and AFLP Analysis of Diploid Cardamine (Brassicaceae) Taxa from Closely Related Polyploid Complexes
Ann. Bot., May 1, 2004; 93(5): 507 - 520.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
M. Schein, Z. Yang, T. Mitchell-Olds, and K. J. Schmid
Rapid Evolution of a Pollen-Specific Oleosin-Like Gene Family from Arabidopsis thaliana and Closely Related Species
Mol. Biol. Evol., April 1, 2004; 21(4): 659 - 669.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.