MBE Advance Access originally published online on June 27, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Mol. Biol. Evol. 20(10):1650-1658. 2003
DOI: 10.1093/molbev/msg182
© 2003 by the Society for Molecular Biology and Evolution. ISSN: 0737-4038
Selection-Driven Evolution of Emergent Dengue Virus
,
* Department of Biology, University of Puerto Rico–Rio Piedras, San Juan, Puerto Rico
Department of Zoology, University of Oxford, Oxford, England
Department of Microbiology and Medical Zoology, University of Puerto Rico–Ciencias Medicas, San Juan, Puerto Rico
Centers for Disease Control and Prevention, San Juan Branch, San Juan, Puerto Rico
|| Centers for Disease Control and Prevention, Fort Collins, Colorado
E-mail: sbennett{at}rrpac.upr.clu.edu.
In the last four decades the incidence of dengue fever has increased 30-fold worldwide, and over half the world's population is now threatened with infection from one or more of four co-circulating viral serotypes (DEN-1 through DEN-4). To determine the role of viral molecular evolution in emergent disease dynamics, we sequenced 40% of the genome of 82 DEN-4 isolates collected from Puerto Rico over the 20 years since the onset of endemic dengue on the island. Isolates were derived from years with varying levels of DEN-4 prevalence. Over our sampling period there were marked evolutionary shifts in DEN-4 viral populations circulating in Puerto Rico; viral lineages were temporally clustered and the most common genotype at a particular sampling time often arose from a previously rare lineage. Expressed changes in structural genes did not appear to drive this lineage turnover, even though these regions include primary determinants of viral antigenic properties. Instead, recent dengue evolution can be attributed in part to positive selection on the nonstructural gene 2A (NS2A), whose functions may include replication efficiency and antigenicity. During the latest and most severe DEN-4 epidemic in Puerto Rico, in 1998, viruses were distinguished by three amino acid changes in NS2A that were fixed far faster than expected by drift alone. Our study therefore demonstrates viral genetic turnover within a focal population and the potential importance of adaptive evolution in viral epidemic expansion.
Key Words: dengue virus • positive selection • epidemiology • phylogeny • maximum likelihood
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. G. Jarman, E. C. Holmes, P. Rodpradit, C. Klungthong, R. V. Gibbons, A. Nisalak, A. L. Rothman, D. H. Libraty, F. A. Ennis, M. P. Mammen Jr., et al. Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand J. Virol., June 1, 2008; 82(11): 5494 - 5500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nagao and K. Koelle Decreases in dengue transmission may act to increase the incidence of dengue hemorrhagic fever PNAS, February 12, 2008; 105(6): 2238 - 2243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Aaskov, K. Buzacott, E. Field, K. Lowry, A. Berlioz-Arthaud, and E. C. Holmes Multiple recombinant dengue type 1 viruses in an isolate from a dengue patient J. Gen. Virol., December 1, 2007; 88(12): 3334 - 3340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Lai, A. P. Goncalvez, R. Men, C. Wernly, O. Donau, R. E. Engle, and R. H. Purcell Epitope Determinants of a Chimpanzee Dengue Virus Type 4 (DENV-4)-Neutralizing Antibody and Protection against DENV-4 Challenge in Mice and Rhesus Monkeys by Passively Transferred Humanized Antibody J. Virol., December 1, 2007; 81(23): 12766 - 12774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. McELROY, K. A. TSETSARKIN, D. L. VANLANDINGHAM, and S. HIGGS MANIPULATION OF THE YELLOW FEVER VIRUS NON-STRUCTURAL GENES 2A AND 4B AND THE 3'NON-CODING REGION TO EVALUATE GENETIC DETERMINANTS OF VIRAL DISSEMINATION FROM THE AEDES AEGYPTI MIDGUT Am J Trop Med Hyg, December 1, 2006; 75(6): 1158 - 1164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Adams, E. C. Holmes, C. Zhang, M. P. Mammen Jr, S. Nimmannitya, S. Kalayanarooj, and M. Boots Cross-protective immunity can account for the alternating epidemic pattern of dengue virus serotypes circulating in Bangkok PNAS, September 19, 2006; 103(38): 14234 - 14239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhang, M. P. Mammen Jr, P. Chinnawirotpisan, C. Klungthong, P. Rodpradit, A. Nisalak, D. W. Vaughn, S. Nimmannitya, S. Kalayanarooj, and E. C. Holmes Structure and age of genetic diversity of dengue virus type 2 in Thailand. J. Gen. Virol., April 1, 2006; 87(Pt 4): 873 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Bennett, E. C. Holmes, M. Chirivella, D. M. Rodriguez, M. Beltran, V. Vorndam, D. J. Gubler, and W. O. McMillan Molecular evolution of dengue 2 virus in Puerto Rico: positive selection in the viral envelope accompanies clade reintroduction. J. Gen. Virol., April 1, 2006; 87(Pt 4): 885 - 893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhang, M. P. Mammen Jr., P. Chinnawirotpisan, C. Klungthong, P. Rodpradit, P. Monkongdee, S. Nimmannitya, S. Kalayanarooj, and E. C. Holmes Clade Replacements in Dengue Virus Serotypes 1 and 3 Are Associated with Changing Serotype Prevalence J. Virol., December 15, 2005; 79(24): 15123 - 15130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. F. Carrington, J. E. Foster, O. G. Pybus, S. N. Bennett, and E. C. Holmes Invasion and Maintenance of Dengue Virus Type 2 and Type 4 in the Americas J. Virol., December 1, 2005; 79(23): 14680 - 14687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kuno and G.-J. J. Chang Biological Transmission of Arboviruses: Reexamination of and New Insights into Components, Mechanisms, and Unique Traits as Well as Their Evolutionary Trends Clin. Microbiol. Rev., October 1, 2005; 18(4): 608 - 637. [Abstract] [Full Text] [PDF]
|
|