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Molecular Biology and Evolution, Vol 2, 399-410, Copyright © 1985 by Society for Molecular Biology and Evolution


ORIGINAL ARTICLE

The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata

HJ Elwood, GJ Olsen and ML Sogin
Department of Molecular and Cellular Biology, National Jewish Hospital, Denver, Colorado 80206.

We have determined the complete nucleotide sequence of the small- subunit ribosomal RNA genes for the ciliate protozoans Stylonychia pustulata and Oxytricha nova. The sequences are homologous and sufficiently similar that these organisms must be closely related. In a phylogeny inferred from comparisons of several eukaryotic small-subunit ribosomal RNAs, the divergence of the ciliates from the eukaryotic line of descent is seen to coincide with the radiation of the plants, the animals, and the fungi. This radiation is preceded by the divergence of the slime mold, Dictyostelium discoideum.
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