Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Palacios, C.
Right arrow Articles by Wernegreen, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palacios, C.
Right arrow Articles by Wernegreen, J. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Biology and Evolution 19:1575-1584 (2002)
© 2002 Society for Molecular Biology and Evolution

A Strong Effect of AT Mutational Bias on Amino Acid Usage in Buchnera is Mitigated at High-Expression Genes

Carmen Palacios and Jennifer J. Wernegreen

Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts

The advent of full genome sequences provides exceptionally rich data sets to explore molecular and evolutionary mechanisms that shape divergence among and within genomes. In this study, we use multivariate analysis to determine the processes driving genome-wide patterns of amino usage in the obligate endosymbiont Buchnera and its close free-living relative Escherichia coli. In the AT-rich Buchnera genome, the primary source of variation in amino acid usage differentiates high- and low-expression genes. Amino acids of high-expression Buchnera genes are generally less aromatic and use relatively GC-rich codons, suggesting that selection against aromatic amino acids and against amino acids with AT-rich codons is stronger in high-expression genes. Selection to maintain hydrophobic amino acids in integral membrane proteins is a primary factor driving protein evolution in E. coli but is a secondary factor in Buchnera. In E. coli, gene expression is a secondary force driving amino acid usage, and a correlation with tRNA abundance suggests that translational selection contributes to this effect. Although this and previous studies demonstrate that AT mutational bias and genetic drift influence amino acid usage in Buchnera, this genome-wide analysis argues that selection is sufficient to affect the amino acid content of proteins with different expression and hydropathy levels.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
J. Hu and J. L. Blanchard
Environmental Sequence Data from the Sargasso Sea Reveal That the Characteristics of Genome Reduction in Prochlorococcus Are Not a Harbinger for an Escalation in Genetic Drift
Mol. Biol. Evol., May 1, 2009; 26(5): 1191 - 1191.
[Full Text] [PDF]

Home page
 MicrobiologyHome page
J. Bodilis and S. Barray
Molecular evolution of the major outer-membrane protein gene (oprF) of Pseudomonas.
Microbiology, April 1, 2006; 152(Pt 4): 1075 - 1088.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
E. Belda, A. Moya, and F. J. Silva
Genome Rearrangement Distances and Gene Order Phylogeny in {gamma}-Proteobacteria
Mol. Biol. Evol., June 1, 2005; 22(6): 1456 - 1467.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
J. T. Herbeck, P. H. Degnan, and J. J. Wernegreen
Nonhomogeneous Model of Sequence Evolution Indicates Independent Origins of Primary Endosymbionts Within the Enterobacteriales ({gamma}-Proteobacteria)
Mol. Biol. Evol., March 1, 2005; 22(3): 520 - 532.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
B. Canback, I. Tamas, and S. G. E. Andersson
A Phylogenomic Study of Endosymbiotic Bacteria
Mol. Biol. Evol., June 1, 2004; 21(6): 1110 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
C. Rispe, F. Delmotte, R. C.H.J. van Ham, and A. Moya
Mutational and Selective Pressures on Codon and Amino Acid Usage in Buchnera, Endosymbiotic Bacteria of Aphids
Genome Res., January 1, 2004; 14(1): 44 - 53.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. T. Herbeck, D. J. Funk, P. H. Degnan, and J. J. Wernegreen
A Conservative Test of Genetic Drift in the Endosymbiotic Bacterium Buchnera: Slightly Deleterious Mutations in the Chaperonin groEL
Genetics, December 1, 2003; 165(4): 1651 - 1660.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. T. Herbeck, D. P. Wall, and J. J. Wernegreen
Gene expression level influences amino acid usage, but not codon usage, in the tsetse fly endosymbiont Wigglesworthia
Microbiology, September 1, 2003; 149(9): 2585 - 2596.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
M. Woolfit and L. Bromham
Increased Rates of Sequence Evolution in Endosymbiotic Bacteria and Fungi with Small Effective Population Sizes
Mol. Biol. Evol., September 1, 2003; 20(9): 1545 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
H. Akashi
Translational Selection and Yeast Proteome Evolution
Genetics, August 1, 2003; 164(4): 1291 - 1303.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Bull.Home page
J. J. Wernegreen, P. H. Degnan, A. B. Lazarus, C. Palacios, and S. R. Bordenstein
Genome Evolution in an Insect Cell: Distinct Features of an Ant-Bacterial Partnership
Biol. Bull., April 1, 2003; 204(2): 221 - 231.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.