Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Roehrdanz, R. L.
Right arrow Articles by Black, W. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roehrdanz, R. L.
Right arrow Articles by Black, W. C., IV
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Biology and Evolution 19:841-849 (2002)
© 2002 Society for Molecular Biology and Evolution

Novel Rearrangements of Arthropod Mitochondrial DNA Detected with Long-PCR: Applications to Arthropod Phylogeny and Evolution

R. L. Roehrdanz2, M. E. Degrugillier and W. C. Black, IV

*Biosciences Research Laboratory, Red River Valley Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Fargo;
{dagger}Department of Microbiology, Colorado State University

Rearrangements of mitochondrial DNA gene order have been suggested as a tool for defining the pattern of evolutionary divergence in arthropod taxa. We have employed a combination of highly conserved insect-based polymerase chain reaction (PCR) primers with long-PCR to survey 14 noninsect arthropods for mitochondrial gene rearrangements. The size of the amplified fragments was used to order the primer containing genes. Five chelicerates exhibit amplicons that are consistent with the presumptive ancestral arthropod mtDNA gene order. These five species comprise two soft ticks, two prostriate hard ticks, and an opilionid. Six other chelicerates, all metastriate hard ticks, have a different arrangement that was originally discovered by this procedure and has been previously detailed in a complete mtDNA sequence. Three new arthropod mtDNA gene arrangements are described here. They were discovered in a terrestrial crustacean (Isopoda) and two myriapods (Chilopoda, centipede; Diplopoda, millipede). These rearrangements include major realignments of some of the large coding regions and two possible new positions for the tRNAMet (M) gene in arthropods. The long-PCR approach affords an opportunity to quickly screen divergent taxa for major rearrangements. Taxa exhibiting rearrangements can be targeted for DNA sequencing of gene boundaries to establish the details of the mtDNA organization.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J PLANKTON RESHome page
C. D. Todd
The copepod parasite (Lepeophtheirus salmonis (Kroyer), Caligus elongatus Nordmann) interactions between wild and farmed Atlantic salmon (Salmo salar L.) and wild sea trout (Salmo trutta L.): a mini review
J. Plankton Res., March 1, 2007; 29(suppl_1): i61 - i71.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
K. D. E. Everett, M. Thao, M. Horn, G. E. Dyszynski, and P. Baumann
Novel chlamydiae in whiteflies and scale insects: endosymbionts 'Candidatus Fritschea bemisiae' strain Falk and 'Candidatus Fritschea eriococci' strain Elm
Int J Syst Evol Microbiol, July 1, 2005; 55(4): 1581 - 1587.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
S. E. Masta and J. L. Boore
The Complete Mitochondrial Genome Sequence of the Spider Habronattus oregonensis Reveals Rearranged and Extremely Truncated tRNAs
Mol. Biol. Evol., May 1, 2004; 21(5): 893 - 902.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
E. Negrisolo, A. Minelli, and G. Valle
The Mitochondrial Genome of the House Centipede Scutigera and the Monophyly Versus Paraphyly of Myriapods
Mol. Biol. Evol., April 1, 2004; 21(4): 770 - 780.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Jameson, A. P. Gibson, C. Hudelot, and P. G. Higgs
OGRe: a relational database for comparative analysis of mitochondrial genomes
Nucleic Acids Res., January 1, 2003; 31(1): 202 - 206.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.