Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow sequence alignment for Figure 6
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Casaregola, S.
Right arrow Articles by Gaillardin, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casaregola, S.
Right arrow Articles by Gaillardin, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Biology and Evolution 19:664-677 (2002)
© 2002 Society for Molecular Biology and Evolution

Ylli, a Non–LTR Retrotransposon L1 Family in the Dimorphic Yeast Yarrowia lipolytica

Serge Casaregola3, Cécile Neuvéglise, Elisabeth Bon and Claude Gaillardin

Collection de Levures d'Intérêt Biotechnologique, Laboratoire de Génétique Moleculaire et Cellulaire, Thiverval-Grignon, France

During the course of a random sequencing project of the genome of the dimorphic yeast Yarrowia lipolytica, we have identified sequences that were repeated in the genome and that matched the reverse transcriptase (RT) sequence of non–long terminal repeat (non–LTR) retrotransposons. Extension of sequencing on each side of this zone of homology allowed the definition of an element over 6 kb long. The conceptual translation of this sequence revealed two open reading frames (ORFs) that displayed several characteristics of non–LTR retrotransposons: a Cys-rich motif in the ORF1, an N-terminal endonuclease, a central RT, and a C-terminal zinc finger domain in the ORF2. We called this element Ylli (for Y. lipolytica LINE). A total of 19 distinct repeats carrying the 3' untranslated region (UTR) and all ending with a poly-A tail were detected. Most of them were very short, 17 being 134 bp long or less. The number of copies of Ylli was estimated to be around 100 if these short repeats are 5' truncations. No 5' UTR was clearly identified, indicating that entire and therefore active elements might be very rare in the Y. lipolytica strain tested. Ylli does not seem to have any insertion specificity. Phylogenetic analysis of the RT domain unambiguously placed Ylli within the L1 clade. It forms a monophyletic group with the Zorro non–LTR retrotransposons discovered in another dimorphic yeast Candida albicans. BLAST comparisons showed that ORF2 of Ylli is closely related to that of the slime mold Dictyostelium discoideum L1 family, TRE.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
M. Woolfit, E. Rozpedowska, J. Piskur, and K. H. Wolfe
Genome Survey Sequencing of the Wine Spoilage Yeast Dekkera (Brettanomyces) bruxellensis
Eukaryot. Cell, April 1, 2007; 6(4): 721 - 733.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
C. Neuveglise, F. Chalvet, P. Wincker, C. Gaillardin, and S. Casaregola
Mutator-Like Element in the Yeast Yarrowia lipolytica Displays Multiple Alternative Splicings
Eukaryot. Cell, March 1, 2005; 4(3): 615 - 624.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Bon, S. Casaregola, G. Blandin, B. Llorente, C. Neuveglise, M. Munsterkotter, U. Guldener, H.-W. Mewes, J. V. Helden, B. Dujon, et al.
Molecular evolution of eukaryotic genomes: hemiascomycetous yeast spliceosomal introns
Nucleic Acids Res., February 15, 2003; 31(4): 1121 - 1135.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.