Molecular Biology and Evolution 17:1661-1672 (2000)
© 2000 Society for Molecular Biology and Evolution
ARTICLE |
Ancestral Exonic Organization of FUT8, the Gene Encoding the 
6-Fucosyltransferase, Reveals Successive Peptide Domains Which Suggest a Particular Three-Dimensional Core Structure for the 6-Fucosyltransferase Family
*Unité de Génétique Moléculaire Animale-UR 1061 (INRA/Université de Limoges), Institut des Sciences de la Vie et de la Santé, Faculté des Sciences, Limoges, France;
UPRES1074, Glycobiologie et Biotechnologie, Faculté des Sciences, Limoges, France;
Centre National de la Recherche Scientifique, Laboratoire de Chimie Biologique, Villeneuve d'Ascq, France;
and
§Glycobiologie INSERM U 504/Université Paris Sud XI, Villejuif, France
Based on PCR strategies and expression studies, we define the genomic organization of the FUT8b gene. This gene encodes the only known mammalian enzyme transferring fucose in an 
1
6 linkage on the asparagine-branched GlcNAc residue of the chitobiose unit of complex N-glycans. The intron/exon organization of the bovine coding sequence determines five successive functional domains. The first exon encodes a domain homologous to cytoskeleton proteins, the second presents a proline-rich region including a motif XPXPPYXP similar to the peptide ligand of the SH3-domain proteins, the third encodes a gyrase-like domain (an enzyme which can bind nucleotides), and the fourth encodes a peptide sequence homologous to the catalytic domain of proteins transferring sugars. Finally, the last exon encodes a domain homologous to the SH3 conserved motif of the SH2-SH3 protein family. This organization suggests that intramolecular interactions might give a tulip-shaped scaffolding, including the catalytic pocket of the enzyme in the Golgi lumen. Deduced from the published sequence of chromosome 14 (AL109847), the human gene organization of FUT8 seems to be similar to that of bovine FUT8b, although the exon partition is more pronounced (bovine exons 1 and 2 correspond to human exons 1–6). The mosaicism and phylogenetic positions of the 6-fucosyltransferase genes are compared with those of other fucosyltransferase genes.
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