Molecular Biology and Evolution, Vol 16, 1280-1291, Copyright © 1999 by Society for Molecular Biology and Evolution
DS Horner, RP Hirt and TM Embley
The iron sulfur protein pyruvate: ferredoxin oxidoreductase (PFO) is
central to energy metabolism in amitochondriate eukaryotes, including those
with hydrogenosomes. Thus, revealing the evolutionary history of PFO is
critical to understanding the origin(s) of eukaryote anaerobic energy
metabolism. We determined a complete PFO sequence for Spironucleus
barkhanus, a large fragment of a PFO sequence from Clostridium
pasteurianum, and a fragment of a new PFO from Giardia lamblia.
Phylogenetic analyses of eubacterial and eukaryotic PFO genes suggest a
complex history for PFO, including possible gene duplications and
horizontal transfers among eubacteria. Our analyses favor a common origin
for eukaryotic cytosolic and hydrogenosomal PFOs from a single eubacterial
source, rather than from separate horizontal transfers as previously
suggested. However, with the present sampling of genes and species, we were
unable to infer a specific eubacterial sister group for eukaryotic PFO.
Thus, we find no direct support for the published hypothesis that the donor
of eukaryote PFO was the common alpha- proteobacterial ancestor of
mitochondria and hydrogenosomes. We also report that several fungi and
protists encode proteins with PFO domains that are likely monophyletic with
PFOs from anaerobic protists. In Saccharomyces cerevisiae, PFO domains
combine with fragments of other redox proteins to form fusion proteins
which participate in methionine biosynthesis. Our results are consistent
with the view that PFO, an enzyme previously considered to be specific to
energy metabolism in amitochondriate protists, was present in the common
ancestor of contemporary eukaryotes and was retained, wholly or in part,
during the evolution of oxygen-dependent and mitochondrion-bearing
lineages.
ORIGINAL ARTICLE
A single eubacterial origin of eukaryotic pyruvate: ferredoxin oxidoreductase genes: implications for the evolution of anaerobic eukaryotes
Department of Zoology, Natural History Museum, London, England.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Muller, S. Ley, I. Felger, A. Hemphill, and N. Muller Identification of differentially expressed genes in a Giardia lamblia WB C6 clone resistant to nitazoxanide and metronidazole J. Antimicrob. Chemother., July 1, 2008; 62(1): 72 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Muller, M. Sterk, A. Hemphill, and N. Muller Characterization of Giardia lamblia WB C6 clones resistant to nitazoxanide and to metronidazole J. Antimicrob. Chemother., August 1, 2007; 60(2): 280 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. St. Maurice, N. Cremades, M. A. Croxen, G. Sisson, J. Sancho, and P. S. Hoffman Flavodoxin:Quinone Reductase (FqrB): a Redox Partner of Pyruvate:Ferredoxin Oxidoreductase That Reversibly Couples Pyruvate Oxidation to NADPH Production in Helicobacter pylori and Campylobacter jejuni J. Bacteriol., July 1, 2007; 189(13): 4764 - 4773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Muller, J. Wastling, S. Sanderson, N. Muller, and A. Hemphill A Novel Giardia lamblia Nitroreductase, GlNR1, Interacts with Nitazoxanide and Other Thiazolides Antimicrob. Agents Chemother., June 1, 2007; 51(6): 1979 - 1986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Hoffman, G. Sisson, M. A. Croxen, K. Welch, W. D. Harman, N. Cremades, and M. G. Morash Antiparasitic Drug Nitazoxanide Inhibits the Pyruvate Oxidoreductases of Helicobacter pylori, Selected Anaerobic Bacteria and Parasites, and Campylobacter jejuni Antimicrob. Agents Chemother., March 1, 2007; 51(3): 868 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Ali and T. Nozaki Current Therapeutics, Their Problems, and Sulfur-Containing-Amino-Acid Metabolism as a Novel Target against Infections by "Amitochondriate" Protozoan Parasites Clin. Microbiol. Rev., January 1, 2007; 20(1): 164 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Martin Embley Multiple secondary origins of the anaerobic lifestyle in eukaryotes Phil Trans R Soc B, June 29, 2006; 361(1470): 1055 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Atteia, R. van Lis, G. Gelius-Dietrich, A. Adrait, J. Garin, J. Joyard, N. Rolland, and W. Martin Pyruvate Formate-lyase and a Novel Route of Eukaryotic ATP Synthesis in Chlamydomonas Mitochondria J. Biol. Chem., April 14, 2006; 281(15): 9909 - 9918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Muller, G. Ruhle, N. Muller, J.-F. Rossignol, and A. Hemphill In Vitro Effects of Thiazolides on Giardia lamblia WB Clone C6 Cultured Axenically and in Coculture with Caco2 Cells Antimicrob. Agents Chemother., January 1, 2006; 50(1): 162 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sutak, P. Dolezal, H. L. Fiumera, I. Hrdy, A. Dancis, M. Delgadillo-Correa, P. J. Johnson, M. Muller, and J. Tachezy From the Cover: Mitochondrial-type assembly of FeS centers in the hydrogenosomes of the amitochondriate eukaryote Trichomonas vaginalis PNAS, July 13, 2004; 101(28): 10368 - 10373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Theissen, M. Hoffmeister, M. Grieshaber, and W. Martin Single Eubacterial Origin of Eukaryotic Sulfide:Quinone Oxidoreductase, a Mitochondrial Enzyme Conserved from the Early Evolution of Eukaryotes During Anoxic and Sulfidic Times Mol. Biol. Evol., September 1, 2003; 20(9): 1564 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. J. Nixon, J. Field, A. G. McArthur, M. L. Sogin, N. Yarlett, B. J. Loftus, and J. Samuelson Iron-Dependent Hydrogenases of Entamoeba histolytica and Giardia lamblia: Activity of the Recombinant Entamoebic Enzyme and Evidence for Lateral Gene Transfer Biol. Bull., February 1, 2003; 204(1): 1 - 9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Silberman, A. G. B. Simpson, J. Kulda, I. Cepicka, V. Hampl, P. J. Johnson, and A. J. Roger Retortamonad Flagellates are Closely Related to Diplomonads--Implications for the History of Mitochondrial Function in Eukaryote Evolution Mol. Biol. Evol., May 1, 2002; 19(5): 777 - 786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. J. Nixon, A. Wang, J. Field, H. G. Morrison, A. G. McArthur, M. L. Sogin, B. J. Loftus, and J. Samuelson Evidence for Lateral Transfer of Genes Encoding Ferredoxins, Nitroreductases, NADH Oxidase, and Alcohol Dehydrogenase 3 from Anaerobic Prokaryotes to Giardialamblia and Entamoebahistolytica Eukaryot. Cell, April 1, 2002; 1(2): 181 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Horner and T. M. Embley Chaperonin 60 Phylogeny Provides Further Evidence for Secondary Loss of Mitochondria Among Putative Early-Branching Eukaryotes Mol. Biol. Evol., October 1, 2001; 18(10): 1970 - 1975. [Full Text] [PDF]
|
|
|
|
|
|
C. Rotte, F. Stejskal, G. Zhu, J. S. Keithly, and W. Martin Pyruvate : NADP Oxidoreductase from the Mitochondrion of Euglena gracilis and from the Apicomplexan Cryptosporidium parvum: A Biochemical Relic Linking Pyruvate Metabolism in Mitochondriate and Amitochondriate Protists Mol. Biol. Evol., May 1, 2001; 18(5): 710 - 720. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. G. Kurland and S. G. E. Andersson Origin and Evolution of the Mitochondrial Proteome Microbiol. Mol. Biol. Rev., December 1, 2000; 64(4): 786 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Horner, P. G. Foster, and T. M. Embley Iron Hydrogenases and the Evolution of Anaerobic Eukaryotes Mol. Biol. Evol., November 1, 2000; 17(11): 1695 - 1709. [Abstract] [Full Text] [PDF]
|
|