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Molecular Biology and Evolution, Vol 16, 760-772, Copyright © 1999 by Society for Molecular Biology and Evolution
Z Tu
Five short interspersed repetitive elements (SINEs) were found fortuitously
in the introns of a steroid hormone receptor AaHR3-2 gene of the yellow
fever mosquito, Aedes aegypti, constituting a novel family of tRNA-related
SINEs named Feilai. In addition, nine other Feilai elements were found in
currently available sequences in Ae. aegypti, six of which were also near
genes. Approximately 5.9 x 10(4) copies of Feilai were present in Ae.
aegypti, equivalent to 2% of the entire genome. An additional 35 Feilai
elements were isolated from a genomic library. Of the total 49 Feilai
elements, 20 were full-length. Sequence comparisons and phylogenetic
analyses of the full-length elements strongly suggest that there are at
least two subfamilies within the Feilai family. There is a high degree of
conservation within the two subfamilies. However, sequence divergence
between the subfamilies, along with the presence of highly degenerate
Feilai elements, suggests that Feilai is likely a diverse family of SINEs
that has existed in Ae. aegypti for a long time. Many Feilai elements were
closely associated with other transposons, especially with fragments of
non-LTR retrotransposons and miniature inverted-repeat transposable
elements. The 500-bp sequences immediately flanking a Feilai element were
highly A + T-rich, which is consistent with the fact that no Feilai has
been found in the coding regions of genes. It is likely that the highly
reiterated and interspersed Feilai elements are partially responsible for
the pattern of short-period interspersion of the Ae. aegypti genome. The
evolutionary relationship between Feilai and the Ae. aegypti genome is
likely complex.
ORIGINAL ARTICLE
Genomic and evolutionary analysis of Feilai, a diverse family of highly reiterated SINEs in the yellow fever mosquito, Aedes aegypti
Department of Entomology, University of Arizona, USA. jaketu@ag.arizona.edu
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