Molecular Biology and Evolution, Vol 16, 1496-1502, Copyright © 1999 by Society for Molecular Biology and Evolution
EJ Feil, MC Maiden, M Achtman and BG Spratt
Multilocus sequence typing (MLST) is a recently developed nucleotide
sequence-based method for the definitive assignment of isolates within
bacterial populations to specific clones. MLST uses the same principles as
multilocus enzyme electrophoresis and provides data that can be used to
investigate aspects of the population genetics and evolution of bacterial
species. We used an MLST data set consisting of the sequences of
approximately 450-bp fragments from seven housekeeping loci from a large
strain collection of Neisseria meningitidis to estimate the relative impact
of recombination compared with point mutation in the diversification of N.
meningitidis clonal complexes. 126 meningococcal isolates were assigned to
10 clonal complexes, 9 of which contained minor clonal variants. The
allelic variation within each complex was classified as a recombinational
exchange or a putative point mutation through a comparison of the sequences
of each variant allele with that of the allele typically found in the
clonal complex. The nine clonal complexes contained a total of 23 allelic
variants, and analysis of the sequences of these variant alleles revealed
that a single nucleotide site in a meningococcal housekeeping gene is at
least 80-fold more likely to change as a result of recombination than as a
result of mutation. This value is estimated to be 10-50-fold for
Escherichia coli and approximately 50-fold for Streptococcus pneumoniae.
ORIGINAL ARTICLE
The relative contributions of recombination and mutation to the divergence of clones of Neisseria meningitidis
Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, England. ed.feil@ceid.ox.ac.uk
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