Molecular Biology and Evolution, Vol 15, 552-559, Copyright © 1998 by Society for Molecular Biology and Evolution
D Charlesworth, FL Liu and L Zhang
We present results showing that several species in the plant genus
Leavenworthia, in the Brassica family, have three alcohol dehydrogenase
loci, unlike Arabidopsis thaliana, which has only a single classical (class
P) alcohol dehydrogenase locus. Based on a portion of the sequence, the
alcohol dehydrogenase loci of Leavenworthia show about 92%-93% amino acid
sequence identity to that of the A. thaliana alcohol dehydrogenase. The
great majority of the sequence differences from the A. thaliana Adh-coding
sequence, and also between three different Leavenworthia species, are
synonymous, suggesting that all are currently functional (or have been in
the recent evolutionary past). The loci differ in the numbers of introns
present, with one locus (Adh- 3) having no introns present. RT-PCR tests
detect expression of all three loci. Linkage data using variant alleles
identified by single- strand conformation polymorphism analysis show that
the three Leavenworthia loci are not closely linked. The results therefore
suggest that the Adh-3 locus may have arisen via an mRNA intermediate but,
despite loss of the introns, is expressed.
ORIGINAL ARTICLE
The evolution of the alcohol dehydrogenase gene family by loss of introns in plants of the genus Leavenworthia (Brassicaceae)
Department of Ecology and Evolution, University of Chicago, USA. deborah.charlesworth@ed.ac.uk
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