Molecular Biology and Evolution, Vol 15, 1275-1287, Copyright © 1998 by Society for Molecular Biology and Evolution
R Peakall, S Gilmore, W Keys, M Morgante and A Rafalski
We investigated the transferability of 31 soybean (Glycine max) simple
sequence repeat (SSR) loci to wild congeners and to other legume genera. Up
to 65% of the soybean primer pairs amplified SSRs within Glycine, but
frequently, the SSRs were short and interrupted compared with those of
soybeans. Nevertheless, 85% of the loci were polymorphic within G.
clandestina. Cross-species amplification outside of the genus was much
lower (3%-13%), with polymorphism restricted to one primer pair, AG81. AG81
amplified loci in Glycine, Kennedia, and Vigna (Phaseoleae), Vicia
(Vicieae), Trifolium (Trifolieae), and Lupinus (Genisteae) within the
Papilionoideae, and in Albizia within the Mimosoideae. The primer
conservation at AG81 may be explained by its apparent proximity to the
seryl-tRNA synthetase gene. Interspecific differences in allele size at
AG81 loci reflected repeat length variation within the SSR region and
indels in the flanking region. Alleles of identical size with different
underlying sequences (size homoplasy) were observed. Our findings and the
emerging patterns in other plant studies suggest that in contrast to
animals, successful cross-species amplification of SSRs in plants is
largely restricted to congeners or closely related genera. Because
mutations in both the SSR region and the flanking region contribute to
variation in allele size among species, knowledge of DNA sequence is
essential before SSR loci can be meaningfully used to address applied and
evolutionary questions.
ORIGINAL ARTICLE
Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants
Division of Botany and Zoology, Australian National University, Canberra, Australia. rod.peakall@anu.edu.au
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