Molecular Biology and Evolution, Vol 13, 605-611, Copyright © 1996 by Society for Molecular Biology and Evolution
T Sitnikova
Statistical properties of the bootstrap test of interior branch lengths of
phylogenetic trees have been studied and compared with those of the
standard interior-branch test in computer simulations. Examination of the
properties of the tests under the null hypothesis showed that both tests
for an interior branch of a predetermined topology are quite reliable when
the distribution of the branch length estimate approaches a normal
distribution. Unlike the standard interior-branch test, the bootstrap test
appears to retain this property even when the substitution rate varies
among sites. In this case, the distribution of the branch length estimate
deviates from a normal distribution, and the standard interior-branch test
gives conservative confidence probability values. A simple correction
method was developed for both interior- branch tests to be applied for
testing the reliability of tree topologies estimated from sequence data.
This correction for the standard interior-branch test appears to be as
effective as that obtained in our previous study, though it is much
simpler. The bootstrap and standard interior-branch tests for estimated
topologies become conservative as the number of sequence groups in a
star-like tree increases.
ORIGINAL ARTICLE
Bootstrap method of interior-branch test for phylogenetic trees
Institute of Molecular Evolutionary Genetics, Pennsylvania State University, University Park 16802, USA. tanya@imeg.bio.psu.edu
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