Molecular Biology and Evolution, Vol 13, 574-583, Copyright © 1996 by Society for Molecular Biology and Evolution
E Okuyama, H Shibata, H Tachida and T Yamazaki
The nucleotide sequences of the 5'-flanking regions of the duplicated Amy
genes in eight sibling species belonging to the melanogaster species
subgroup are analyzed. In Drosophila melanogaster, a region of about 450 bp
immediately upstream of the translation initiation site of the two
paralogous genes (the proximal and distal genes) has sequence similarities.
However, we could not detect any significant sequence similarity in the
region more upstream than -450. This result indicates that the coding
regions of the ancestral Amy gene were duplicated together with 450 bp of
the 5'-flanking region as one unit. Multiple alignment of these 450-bp
sequences in the proximal and distal genes of all eight species revealed a
mosaic pattern of highly conserved and divergent regions. The conserved
regions included almost all the putative regulatory elements identified in
previous analyses of the sequences. A phylogenetic analysis of the aligned
sequences shows that these 450-bp sequences are clustered into the proximal
and the distal groups. As a whole, the divergence between groups in this
region is very large in contrast to that in the coding regions. Based on
the divergence between groups, the 450-bp region is divided into two
subregions. We found that the ratios of the divergence between groups to
that within groups differ in the two subregions. From these observations,
we discuss a possibility of positive selection acting on the subregion
immediately upstream of the Amy coding region to cause divergence of
regulatory elements of the paralogous genes.
ORIGINAL ARTICLE
Molecular evolution of the 5'-flanking regions of the duplicated Amy genes in Drosophila melanogaster species subgroup
Department of Biology, Kyushu University, Japan.
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