Molecular Biology and Evolution, Vol 13, 549-555, Copyright © 1996 by Society for Molecular Biology and Evolution
AR Lohe and DL Hartl
Genetic studies of the mariner transposable element Mos1 have revealed two
novel types of regulatory mechanisms. In one mechanism, overproduction of
the wild-type transposase reduces the overall level of transposase activity
as assayed by the excision of a nonautonomous mariner target element. This
mechanism is termed overproduction inhibition (OPI). Another mechanism is
observed in a class of hypomorphic missense mutations in the transposase.
In the presence of wild-type Mos1 transposase, these mutations exhibit
dominant-negative complementation (DNC) that antagonizes the activity of
the wild-type transposase. We propose that these regulatory mechanisms act
at the level of the transposase protein subunits by promoting the assembly
of oligomeric forms, or of mixed-subunit oligomers, that have reduced
activity. We suggest that these regulatory mechanisms may apply generally
to mariner-like elements (MLEs). Overproduction inhibition may help explain
why the MLE copy number reaches very different levels in different species.
Dominant-negative complementation may help explain why most naturally
occurring copies of MLEs have been mutationally inactivated.
ORIGINAL ARTICLE
Autoregulation of mariner transposase activity by overproduction and dominant-negative complementation
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA. d_hartl@harvard.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. C.-Y. Wu, Y.-J. J. Meir, C. J. Coates, A. M. Handler, P. Pelczar, S. Moisyadi, and J. M. Kaminski From the Cover: piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells PNAS, October 10, 2006; 103(41): 15008 - 15013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Le Rouzic and P. Capy The First Steps of Transposable Elements Invasion: Parasitic Strategy vs. Genetic Drift Genetics, February 1, 2005; 169(2): 1033 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lipkow, N. Buisine, D. J. Lampe, and R. Chalmers Early Intermediates of mariner Transposition: Catalysis without Synapsis of the Transposon Ends Suggests a Novel Architecture of the Synaptic Complex Mol. Cell. Biol., September 15, 2004; 24(18): 8301 - 8311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. G. Barry, D. J. Witherspoon, and D. J. Lampe A Bacterial Genetic Screen Identifies Functional Coding Sequences of the Insect mariner Transposable Element Famar1 Amplified From the Genome of the Earwig, Forficula auricularia Genetics, February 1, 2004; 166(2): 823 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Petrov, Y. T. Aminetzach, J. C. Davis, D. Bensasson, and A. E. Hirsh Size Matters: Non-LTR Retrotransposable Elements and Ectopic Recombination in Drosophila Mol. Biol. Evol., June 1, 2003; 20(6): 880 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Izsvak, D. Khare, J. Behlke, U. Heinemann, R. H. Plasterk, and Z. Ivics Involvement of a Bifunctional, Paired-like DNA-binding Domain and a Transpositional Enhancer in Sleeping Beauty Transposition J. Biol. Chem., September 6, 2002; 277(37): 34581 - 34588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Lozovsky, D. Nurminsky, E. A. Wimmer, and D. L. Hartl Unexpected Stability of mariner Transgenes in Drosophila Genetics, February 1, 2002; 160(2): 527 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nakayashiki, K. Ikeda, Y. Hashimoto, Y. Tosa, and S. Mayama Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea Nucleic Acids Res., March 15, 2001; 29(6): 1278 - 1284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Hartl Discovery of the Transposable Element Mariner Genetics, February 1, 2001; 157(2): 471 - 476. [Full Text]
|
|
|
|
|
|
L. R. O. Tosi and S. M. Beverley cis and trans factors affecting Mos1 mariner evolution and transposition in vitro, and its potential for functional genomics Nucleic Acids Res., February 1, 2000; 28(3): 784 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Lohe, C. Timmons, I. Beerman, E. R. Lozovskaya, and D. L. Hartl Self-Inflicted Wounds, Template-Directed Gap Repair and a Recombination Hotspot: Effects of the mariner Transposase Genetics, February 1, 2000; 154(2): 647 - 656. [Abstract] [Full Text]
|
|
|
|
|
|
D. J. Lampe, B. J. Akerley, E. J. Rubin, J. J. Mekalanos, and H. M. Robertson Hyperactive transposase mutants of the Himar1 mariner transposon PNAS, September 28, 1999; 96(20): 11428 - 11433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Lampe, T. E. Grant, and H. M. Robertson Factors Affecting Transposition of the Himar1 mariner Transposon in Vitro Genetics, May 1, 1998; 149(1): 179 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Lohe, D. De Aguiar, and D. L. Hartl Mutations in the mariner transposase: The D,D(35)E consensus sequence is nonfunctional PNAS, February 18, 1997; 94(4): 1293 - 1297. [Abstract] [Full Text] [PDF]
|
|