Molecular Biology and Evolution, Vol 12, 1033-1047, Copyright © 1995 by Society for Molecular Biology and Evolution
H Sultmann, WE Mayer, F Figueroa, H Tichy and J Klein
The recent explosive adaptive radiation of cichlids in the great lakes of
Africa has attracted the attention of both morphologists and molecular
biologists. To decipher the phylogenetic relationships among the various
taxa within the family Cichlidae is a prerequisite for answering some
fundamental questions about the nature of the speciation process. In the
present study, we used the random amplification of polymorphic DNA (RAPD)
technique to obtain sequence differences between selected cichlid species.
We then designed specific primers based on these sequences and used them to
amplify template DNA from a large number of species by the polymerase chain
reaction (PCR). We sequenced the amplified products and searched the
sequences for indels and shared substitutions. We identified a number of
such characters at three loci-- DXTU1, DXTU2, and DXTU3--and used them for
phylogenetic and cladistic analysis of the relationships among the various
cichlid groups. Our studies assign an outgroup position to Neotropical
cichlids in relation to African cichlids, provide evidence for a
sister-group relationship of tilapiines to the haplochromines, group
Cyphotilapia frontosa with the lamprologines of Lake Tanganyika, place
Astatoreochromis alluaudi to an outgroup position with respect to other
haplochromines of Lakes Victoria and Malawi, and provide additional support
for the monophyly of the remaining Lake Victoria haplochromines and the
Lake Malawi haplochromines. The described approach holds great promise for
further resolution of cichlid phylogeny.
ORIGINAL ARTICLE
Phylogenetic analysis of cichlid fishes using nuclear DNA markers
Max-Planck-Institut fur Biologie, Tubingen, Germany.
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