Molecular Biology and Evolution, Vol 11, 493-503, Copyright © 1994 by Society for Molecular Biology and Evolution
JJ Beintema, WT Stam, B Hazes and MP Smidt
Crustacean and cheliceratan hemocyanins (oxygen-transport proteins) and
insect hexamerins (storage proteins) are homologous gene products, although
the latter do not bind oxygen and do not possess the copper- binding
histidines present in the hemocyanins. An alignment of 19 amino acid
sequences of hemocyanin subunits and insect hexamerins was made, based on
the conservation of elements of secondary structure observed in X-ray
structures of two hemocyanin subunits. The alignment was analyzed using
parsimony and neighbor-joining methods. Results provide strong indications
for grouping together the sequences of the 2 crustacean hemocyanin
subunits, the 5 cheliceratan hemocyanin subunits, and the 12 insect
hexamerins. Within the insect clade, four methionine- rich proteins, four
arylphorins, and two juvenile hormone-suppressible proteins from
Lepidoptera, as well as two dipteran proteins, form four separate groups.
In the absence of an outgroup sequence, it is not possible to present
information about the ancestral state from which these proteins are
derived. Although this family of proteins clearly consists of homologous
gene products, there remain striking differences in gene organization and
site of biosynthesis of the proteins within the cell. Because studies on
18S and 12S rRNA sequences indicate a rather close relationship between
insects and crustaceans, we propose that hemocyanin is the ancestral
arthropod protein and that insect hexamerins lost their copper-binding
capability after divergence of the insects from the crustaceans.
ORIGINAL ARTICLE
Evolution of arthropod hemocyanins and insect storage proteins (hexamerins)
Department of Biochemistry, University of Groningen, The Netherlands.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. B. Terwilliger, M. Ryan, and M. R. Phillips Crustacean hemocyanin gene family and microarray studies of expression change during eco-physiological stress Integr. Comp. Biol., December 1, 2006; 46(6): 991 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Dolashka-Angelova, A. Dolashki, S. N. Savvides, R. Hristova, J. Van Beeumen, W. Voelter, B. Devreese, U. Weser, P. Di Muro, B. Salvato, et al. Structure of Hemocyanin Subunit CaeSS2 of the Crustacean Mediterranean Crab Carcinus aestuarii J. Biochem., September 1, 2005; 138(3): 303 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. B. Terwilliger, M. C. Ryan, and D. Towle Evolution of novel functions: cryptocyanin helps build new exoskeleton in Cancer magister J. Exp. Biol., July 1, 2005; 208(13): 2467 - 2474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kusche and T. Burmester Diplopod Hemocyanin Sequence and the Phylogenetic Position of the Myriapoda Mol. Biol. Evol., August 1, 2001; 18(8): 1566 - 1573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Burmester Molecular Evolution of the Arthropod Hemocyanin Superfamily Mol. Biol. Evol., February 1, 2001; 18(2): 184 - 195. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Jaenicke, R. Foll, and H. Decker Spider Hemocyanin Binds Ecdysone and 20-OH-Ecdysone J. Biol. Chem., November 26, 1999; 274(48): 34267 - 34271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jaenicke, H. Decker, W. Gebauer, J. Markl, and T. Burmester Identification, Structure, and Properties of Hemocyanins from Diplopod Myriapoda J. Biol. Chem., October 8, 1999; 274(41): 29071 - 29074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Burmester Identification, Molecular Cloning, and Phylogenetic Analysis of a Non-respiratory Pseudo-hemocyanin of Homarus americanus J. Biol. Chem., May 7, 1999; 274(19): 13217 - 13222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. B. Terwilliger, L. Dangott, and M. Ryan Cryptocyanin, a crustacean molting protein: Evolutionary link with arthropod hemocyanins and insect hexamerins PNAS, March 2, 1999; 96(5): 2013 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Decker and T. Rimke Tarantula Hemocyanin Shows Phenoloxidase Activity J. Biol. Chem., October 2, 1998; 273(40): 25889 - 25892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Durstewitz and N. B. Terwilliger Developmental Changes in Hemocyanin Expression in the Dungeness Crab, Cancer magister J. Biol. Chem., February 14, 1997; 272(7): 4347 - 4350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Braun and G. R. Wyatt Sequence of the Hexameric Juvenile Hormone-binding Protein from the Hemolymph of Locusta migratoria J. Biol. Chem., December 6, 1996; 271(49): 31756 - 31762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Voit, G. Feldmaier-Fuchs, T. Schweikardt, H. Decker, and T. Burmester Complete Sequence of the 24-mer Hemocyanin of the Tarantula Eurypelma californicum. STRUCTURE AND INTRAMOLECULAR EVOLUTION OF THE SUBUNITS J. Biol. Chem., December 8, 2000; 275(50): 39339 - 39344. [Abstract] [Full Text] [PDF]
|
|