Molecular Biology and Evolution, Vol 11, 393-405, Copyright © 1994 by Society for Molecular Biology and Evolution
AP Vogler and R DeSalle
Sequence divergence in the internal transcribed spacer region 1 (ITS-1) of
the ribosomal DNA locus was assessed in subspecies of the coastal North
American tiger beetle, Cicindela dorsalis. The spacer region was amplified
using the polymerase chain reaction and cloned for sequencing. Of a total
of 50 clones obtained from 12 specimens, 42 clones were different in at
least one nucleotide position. In a parsimony analysis of these sequences,
the main phylogenetic distinction was found to separate sequences from the
Gulf of Mexico and the Atlantic Ocean. Within these two assemblages
phylogenetic resolution was low, and the variation within individuals was
almost as high as the variation within the entire lineage. The pattern of
sequence variation suggests the existence of two forms of the ITS-1 that
are maintained on different chromosomes. Polymorphisms of limited
geographical distribution could be detected, and 41 additional clones were
partly sequenced, to assess the geographic distribution of these
polymorphisms in more detail. In a population aggregation analysis, the
geographic pattern of ITS-1 distribution was basically congruent with that
obtained in earlier studies from mitochondrial DNA in the same C. dorsalis
populations.
ORIGINAL ARTICLE
Evolution and phylogenetic information content of the ITS-1 region in the tiger beetle Cicindela dorsalis
American Museum of Natural History, Department of Entomology, New York, New York 10024.
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